FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026466523> ?p ?o ?g. }

• W2026466523 endingPage "226" @default.
• W2026466523 startingPage "217" @default.
• W2026466523 abstract "A large-insert bacterial artificial chromosome (BAC) library has been constructed from male chicken genomic DNA using the new pBeloBAC11 vector. The library was prepared in two parts, such that two-thirds of the BAC library (2976 clones) had an average insert size of 490 kb (80 clones analyzed), after optimization of transformation and HMW DNA size-selection conditions. Fragments of increased average size were cloned by coupling a second size strategy using pulsed-field gel electrophoresis with optimized electroporation that favored transformation ofEscherichia coliDH10B cells with very large plasmids. The initial one-third of this library (1440 clones) was constructed using the standard protocols and had an average insert size of 180 kb (40 clones analyzed). The overall library consists, at present, of 4416 clones with a combined insert size average of 390 kb (ranging from 25 to 725 kb). At least 95% of the BAC clones contain inserts. This is partially due to a second color selection performed with respect to white colonies, as well as to the optimized ligation conditions used. Based on the percentage of clones with inserts and the analysis of insert sizes, we estimate this library to represent a 0.8-fold coverage of the chicken genome. Southern blot analysis and fluorescencein situhybridization were performed to confirm the identity of the BAC inserts with chicken genomic DNA. Analysis of large chicken BAC inserts showed that they were stably propagated for at least 120 cell generations. The results indicate that the BAC system is able to carry stably very large genomic fragments of chicken DNA, this system translating into a powerful tool for physical mapping and positional cloning of the chicken genome." @default.
• W2026466523 created "2016-06-24" @default.
• W2026466523 creator A5031036706 @default.
• W2026466523 creator A5053949700 @default.
• W2026466523 date "1997-06-01" @default.
• W2026466523 modified "2023-09-23" @default.
• W2026466523 title "Construction and Characterization of a Large-Fragment Chicken Bacterial Artificial Chromosome Library" @default.
• W2026466523 cites W1121434268 @default.
• W2026466523 cites W1480645851 @default.
• W2026466523 cites W1972372764 @default.
• W2026466523 cites W1976440063 @default.
• W2026466523 cites W1984802151 @default.
• W2026466523 cites W1985318686 @default.
• W2026466523 cites W1988260588 @default.
• W2026466523 cites W1990074059 @default.
• W2026466523 cites W1992943373 @default.
• W2026466523 cites W1993669854 @default.
• W2026466523 cites W1996749102 @default.
• W2026466523 cites W2000678289 @default.
• W2026466523 cites W2005240629 @default.
• W2026466523 cites W2021014064 @default.
• W2026466523 cites W2031117804 @default.
• W2026466523 cites W2036971867 @default.
• W2026466523 cites W2038202952 @default.
• W2026466523 cites W2043542212 @default.
• W2026466523 cites W2049243095 @default.
• W2026466523 cites W2052419428 @default.
• W2026466523 cites W2077003408 @default.
• W2026466523 cites W2079482933 @default.
• W2026466523 cites W2084769556 @default.
• W2026466523 cites W2095714144 @default.
• W2026466523 cites W2152625960 @default.
• W2026466523 cites W2160287678 @default.
• W2026466523 doi "https://doi.org/10.1006/geno.1997.4738" @default.
• W2026466523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9192841" @default.
• W2026466523 hasPublicationYear "1997" @default.
• W2026466523 type Work @default.
• W2026466523 sameAs 2026466523 @default.
• W2026466523 citedByCount "67" @default.
• W2026466523 countsByYear W20264665232012 @default.
• W2026466523 countsByYear W20264665232013 @default.
• W2026466523 countsByYear W20264665232014 @default.
• W2026466523 countsByYear W20264665232015 @default.
• W2026466523 countsByYear W20264665232016 @default.
• W2026466523 countsByYear W20264665232017 @default.
• W2026466523 countsByYear W20264665232019 @default.
• W2026466523 countsByYear W20264665232020 @default.
• W2026466523 countsByYear W20264665232021 @default.
• W2026466523 crossrefType "journal-article" @default.
• W2026466523 hasAuthorship W2026466523A5031036706 @default.
• W2026466523 hasAuthorship W2026466523A5053949700 @default.
• W2026466523 hasConcept C104317684 @default.
• W2026466523 hasConcept C127413603 @default.
• W2026466523 hasConcept C133307689 @default.
• W2026466523 hasConcept C13575015 @default.
• W2026466523 hasConcept C141231307 @default.
• W2026466523 hasConcept C153911025 @default.
• W2026466523 hasConcept C17757408 @default.
• W2026466523 hasConcept C178050479 @default.
• W2026466523 hasConcept C182901222 @default.
• W2026466523 hasConcept C186852380 @default.
• W2026466523 hasConcept C204241405 @default.
• W2026466523 hasConcept C22744801 @default.
• W2026466523 hasConcept C2779656422 @default.
• W2026466523 hasConcept C2780098074 @default.
• W2026466523 hasConcept C3017666073 @default.
• W2026466523 hasConcept C30481170 @default.
• W2026466523 hasConcept C54355233 @default.
• W2026466523 hasConcept C552990157 @default.
• W2026466523 hasConcept C78519656 @default.
• W2026466523 hasConcept C86803240 @default.
• W2026466523 hasConceptScore W2026466523C104317684 @default.
• W2026466523 hasConceptScore W2026466523C127413603 @default.
• W2026466523 hasConceptScore W2026466523C133307689 @default.
• W2026466523 hasConceptScore W2026466523C13575015 @default.
• W2026466523 hasConceptScore W2026466523C141231307 @default.
• W2026466523 hasConceptScore W2026466523C153911025 @default.
• W2026466523 hasConceptScore W2026466523C17757408 @default.
• W2026466523 hasConceptScore W2026466523C178050479 @default.
• W2026466523 hasConceptScore W2026466523C182901222 @default.
• W2026466523 hasConceptScore W2026466523C186852380 @default.
• W2026466523 hasConceptScore W2026466523C204241405 @default.
• W2026466523 hasConceptScore W2026466523C22744801 @default.
• W2026466523 hasConceptScore W2026466523C2779656422 @default.
• W2026466523 hasConceptScore W2026466523C2780098074 @default.
• W2026466523 hasConceptScore W2026466523C3017666073 @default.
• W2026466523 hasConceptScore W2026466523C30481170 @default.
• W2026466523 hasConceptScore W2026466523C54355233 @default.
• W2026466523 hasConceptScore W2026466523C552990157 @default.
• W2026466523 hasConceptScore W2026466523C78519656 @default.
• W2026466523 hasConceptScore W2026466523C86803240 @default.
• W2026466523 hasIssue "2" @default.
• W2026466523 hasLocation W20264665231 @default.
• W2026466523 hasLocation W20264665232 @default.
• W2026466523 hasOpenAccess W2026466523 @default.
• W2026466523 hasPrimaryLocation W20264665231 @default.
• W2026466523 hasRelatedWork W1976675477 @default.
• W2026466523 hasRelatedWork W1988165145 @default.

• next