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- W2026480904 abstract "We developed an efficient method for isolation of novel dnaA mutations based on PCR mutagenesis in the presence of manganese ion and shuffling of dnaA-carrying plasmids in a dnaA deletion host bacterium. Using this system, we obtained 30 cold-sensitive mutants from 4000 clones carrying plasmids with a mutagenized dnaA gene. All 27 cold-sensitive mutants analyzed were defective in DNA replication; none had a DnaAcos (over-initiation) phenotype. Nucleotide sequencing revealed that novel 15 alleles (mutations in 14 amino acid residues) are responsible for the cold-sensitive phenotype and are all located in the carboxy-terminal half of the DnaA protein." @default.
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- W2026480904 date "1999-07-01" @default.
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- W2026480904 title "Isolation and characterization of novel cold-sensitive<i>dnaA</i>mutants of<i>Escherichia coli</i>" @default.
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- W2026480904 doi "https://doi.org/10.1111/j.1574-6968.1999.tb13684.x" @default.
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