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- W2026488959 abstract "A choline oxidase (ChO) immobilized enzyme reactor (IMER) prepared by glutaraldehyde coupling of the enzyme on aminopropyl modified controlled pore glass beads is described. The ChO–IMER was coupled, in a flow injection configuration system, to an interference free hydrogen peroxide amperometric sensor based on a Pt surface modified by an overoxidized polypyrrole film. The resulting analytical device responds selectively to choline and displays a sensitivity of 46.9 ± 0.2 μC mM−1 and a limit of detection, calculated at a signal-to-noise ratio equal to 3, of 7 μM. Sensitivity remains constant for about 20 days and then starts to slowly deteriorate and after 2 months a 70% of the initial sensitivity was still retained. The application to choline determination in milk hydrolysates is demonstrated. Short- and long-term drift observed in the analytical response can be corrected by a bracketing technique." @default.
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- W2026488959 date "2007-07-01" @default.
- W2026488959 modified "2023-10-16" @default.
- W2026488959 title "Flow injection determination of choline in milk hydrolysates by an immobilized enzyme reactor coupled to a selective hydrogen peroxide amperometric sensor" @default.
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- W2026488959 doi "https://doi.org/10.1016/j.aca.2007.05.026" @default.
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