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• W2026558304 abstract "In a previous report from this laboratory (M. Bartkoski and B. Roizman, 1976, J. Virol. 20, 583–588) we reported that herpes simplex virus RNA entering the cytoplasm has a terminal oligonucleotide structure consistent with 7mG(5′)ppp-(5′)XmpYmpNp and that, whereas the viral RNA made early in infection contained methylated mononucleotides, the RNA made late did not. In this paper we report the following: (i) Viral and host adenylated RNA made early in infection were methylated at both the 5′ terminus and internally (6mAp); (ii) in contrast to the RNA made before the onset of synthesis of viral DNA, viral and host adenylated RNA entering the cytoplasm late in infection during the synthesis of γ (structural) polypeptides were methylated at the 5′ terminus but not internally; (iii) the cessation of methylation of internal nucleotides was not due to rapid turnover of the enzyme(s) responsible for this function; (iv) addition of canavanine at any time after infection and until shortly after onset of β-polypeptide synthesis precluded this cessation of internal methylation of host and viral RNA; (v) inhibition of viral DNA synthesis had no effect on the methylation pattern. The role of viral proteins in this inhibition of internal methylation and the significance of the inhibition are discussed." @default.
• W2026558304 created "2016-06-24" @default.
• W2026558304 creator A5011142437 @default.
• W2026558304 creator A5063759652 @default.
• W2026558304 date "1978-03-01" @default.
• W2026558304 modified "2023-09-23" @default.
• W2026558304 title "Regulation of herpesvirus macromolecular synthesis VII. Inhibition of internal methylation of mRNA late in infection" @default.
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• W2026558304 doi "https://doi.org/10.1016/0042-6822(78)90419-1" @default.
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