Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026614454> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2026614454 endingPage "185" @default.
- W2026614454 startingPage "173" @default.
- W2026614454 abstract "The objectives of this research were to investigate conditions for immobilization of L-amino acid oxidase (LAAO) on a preactivated nylon membrane, and to develop an enzyme sensor which can detect ammonia. LAAO was immobilized onto a preactivated nylon membrane. Optimal immobilization conditions were 0.3% glutaraldehyde, 1 mg bovine serum albumin, and 1.6 units of enzyme with 2 h coupling time. The enzymic membrane and an ammonium selective nonactin membrane were attached to an ammonia electrode to fabricate an L-amino acid sensor. L-amino acids were enzymatically degraded by immobilized LAAO, and the ammonia generated was measured by a potentiometric electrode at pH 8.5 and 45C. The sensor showed high relative activities for L-amino acids, and the responses of the sensor for phenylalanine and isoleucine were linear to 10 mM with a detection limit of 0.05 mM. The L-amino acid sensor was applied for monitoring increases in amino acids levels, expressed as L-isoleucine equivalents, during yeast autolysis. Determination of L-amino acids was complete in 3 min. Moreover, the activity of the enzymic membrane was stable for at least 260 assays, and did not noticeably decline for 2 months." @default.
- W2026614454 created "2016-06-24" @default.
- W2026614454 creator A5018697776 @default.
- W2026614454 creator A5066441282 @default.
- W2026614454 date "1999-06-01" @default.
- W2026614454 modified "2023-10-09" @default.
- W2026614454 title "DEVELOPMENT OF A BIOSENSOR WITH IMMOBILIZED L-AMINO ACID OXIDASE FOR DETERMINATION OF L-AMINO ACIDS" @default.
- W2026614454 cites W1972850587 @default.
- W2026614454 cites W1974752982 @default.
- W2026614454 cites W1984179052 @default.
- W2026614454 cites W1985014857 @default.
- W2026614454 cites W1990003795 @default.
- W2026614454 cites W1993114356 @default.
- W2026614454 cites W1995391944 @default.
- W2026614454 cites W1997319852 @default.
- W2026614454 cites W2011922821 @default.
- W2026614454 cites W2020569261 @default.
- W2026614454 cites W2024846792 @default.
- W2026614454 cites W2025210918 @default.
- W2026614454 cites W2027830513 @default.
- W2026614454 cites W2033034930 @default.
- W2026614454 cites W2033378558 @default.
- W2026614454 cites W2038739188 @default.
- W2026614454 cites W2040167594 @default.
- W2026614454 cites W2047966460 @default.
- W2026614454 cites W2053925771 @default.
- W2026614454 cites W2057540746 @default.
- W2026614454 cites W2062156481 @default.
- W2026614454 cites W2063875145 @default.
- W2026614454 cites W2068179848 @default.
- W2026614454 cites W2083693766 @default.
- W2026614454 cites W2084514068 @default.
- W2026614454 cites W2087832674 @default.
- W2026614454 cites W2107638769 @default.
- W2026614454 doi "https://doi.org/10.1111/j.1745-4514.1999.tb00012.x" @default.
- W2026614454 hasPublicationYear "1999" @default.
- W2026614454 type Work @default.
- W2026614454 sameAs 2026614454 @default.
- W2026614454 citedByCount "21" @default.
- W2026614454 countsByYear W20266144542012 @default.
- W2026614454 countsByYear W20266144542013 @default.
- W2026614454 countsByYear W20266144542014 @default.
- W2026614454 countsByYear W20266144542015 @default.
- W2026614454 countsByYear W20266144542017 @default.
- W2026614454 countsByYear W20266144542018 @default.
- W2026614454 countsByYear W20266144542020 @default.
- W2026614454 countsByYear W20266144542023 @default.
- W2026614454 crossrefType "journal-article" @default.
- W2026614454 hasAuthorship W2026614454A5018697776 @default.
- W2026614454 hasAuthorship W2026614454A5066441282 @default.
- W2026614454 hasBestOaLocation W20266144541 @default.
- W2026614454 hasConcept C160756335 @default.
- W2026614454 hasConcept C181199279 @default.
- W2026614454 hasConcept C185592680 @default.
- W2026614454 hasConcept C2777431362 @default.
- W2026614454 hasConcept C2777529624 @default.
- W2026614454 hasConcept C33633552 @default.
- W2026614454 hasConcept C41625074 @default.
- W2026614454 hasConcept C43617362 @default.
- W2026614454 hasConcept C515207424 @default.
- W2026614454 hasConcept C55493867 @default.
- W2026614454 hasConceptScore W2026614454C160756335 @default.
- W2026614454 hasConceptScore W2026614454C181199279 @default.
- W2026614454 hasConceptScore W2026614454C185592680 @default.
- W2026614454 hasConceptScore W2026614454C2777431362 @default.
- W2026614454 hasConceptScore W2026614454C2777529624 @default.
- W2026614454 hasConceptScore W2026614454C33633552 @default.
- W2026614454 hasConceptScore W2026614454C41625074 @default.
- W2026614454 hasConceptScore W2026614454C43617362 @default.
- W2026614454 hasConceptScore W2026614454C515207424 @default.
- W2026614454 hasConceptScore W2026614454C55493867 @default.
- W2026614454 hasIssue "2" @default.
- W2026614454 hasLocation W20266144541 @default.
- W2026614454 hasOpenAccess W2026614454 @default.
- W2026614454 hasPrimaryLocation W20266144541 @default.
- W2026614454 hasRelatedWork W1981229103 @default.
- W2026614454 hasRelatedWork W1999210020 @default.
- W2026614454 hasRelatedWork W1999370887 @default.
- W2026614454 hasRelatedWork W2003189828 @default.
- W2026614454 hasRelatedWork W2037547357 @default.
- W2026614454 hasRelatedWork W2351702666 @default.
- W2026614454 hasRelatedWork W2377144723 @default.
- W2026614454 hasRelatedWork W2779039214 @default.
- W2026614454 hasRelatedWork W4220879675 @default.
- W2026614454 hasRelatedWork W95481140 @default.
- W2026614454 hasVolume "23" @default.
- W2026614454 isParatext "false" @default.
- W2026614454 isRetracted "false" @default.
- W2026614454 magId "2026614454" @default.
- W2026614454 workType "article" @default.