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• W2026651330 abstract "Liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS) was developed for the quantitation of hydromorphone (HYD), an opiate analgesic, in human plasma. A simple liquid-liquid extraction was used to extract the analyte and its deuterated internal standard (d3-HYD). Chromatographic separation of hydromorphone from its metabolite hydromorphone-3-glucuronide (H3G) was necessary because of the significant H3G fragmentation to HYD before Ql of the mass spectrometer, which could result in false detection as HYD in the multiple reaction mode (MRM). This separation was achieved using a 50 x 2 mm, I.D. silica column (5 microm) and a mobile phase of acetonitrile-water formic acid (80:20:1, v/v/v). The method was validated in the concentration range 0.05-10 ng ml(-1) in plasma and met the acceptance criteria of industry guidelines for accuracy, precision, and stability." @default.
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• W2026651330 date "2000-09-01" @default.
• W2026651330 modified "2023-10-16" @default.
• W2026651330 title "Development and validation of a sensitive method for hydromorphone in human plasma by normal phase liquid chromatography–tandem mass spectrometry" @default.
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• W2026651330 doi "https://doi.org/10.1016/s0731-7085(00)00352-6" @default.
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