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- W2026654319 abstract "The unprecedented wealth of databases that have become available in the era of next-generation sequencing has considerably increased our knowledge of bacterial genetic elements (GEs). At the same time, the advent of single-cell based approaches has brought realization that unsuspected heterogeneity may occur in the bacterial population from a single colony. The increasing use of PCR-based techniques to study new GEs requires careful consideration of the possible different PCR targets associated with different subpopulations if incorrect or incomplete interpretations are to be avoided. In this commentary, confining ourselves to our direct experience, we illustrate some examples of PCR pitfalls that may be encountered while investigating GEs." @default.
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- W2026654319 date "2013-05-24" @default.
- W2026654319 modified "2023-09-27" @default.
- W2026654319 title "Pitfalls encountered while investigating genetic elements by PCR" @default.
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- W2026654319 doi "https://doi.org/10.4161/mge.25255" @default.
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