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- W2026681235 abstract "Summary Conditions were established that resulted in high yields and consistent division in 10 -15 of the protoplasts from one to 3 month old mature leaf tissues of Vitis vinifera cv. Koshu Sanjaku grown at 25 °C and 80 % relative humidity without direct sunlight in a greenhouse. Enzymes used for protoplast isolation were the combination of 1 % Macerozyme R-10, 0.01 Pectolyase Y-23, 1 % Cellulase Onozuka RS and 0.4 % Dricelase. Use of a higher concentration of MES buffer than 0.1 M was essential to obtain high yields and viabilities of protoplasts. From these protoplasts new cell walls were regenerated within the first few days and 4 to 6 cell-structures developed from one protoplast by cell division after 9 days of culture in Gamborg's BS liquid medium supplemented with 1.0 mg · 1−1 2,4-D, 0.5 mg · 1−1 6-benzyladenine and 0.5 M sorbitol." @default.
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- W2026681235 date "1985-06-01" @default.
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- W2026681235 title "Cell Regeneration and Division of Grape Mesophyll Protoplasts" @default.
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- W2026681235 doi "https://doi.org/10.1016/s0176-1617(85)80006-7" @default.
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