Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026695979> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W2026695979 endingPage "293" @default.
- W2026695979 startingPage "283" @default.
- W2026695979 abstract "The purpose of the study was to identify genes that are differentially expressed in normal versus keratoconus corneas. Total RNA isolated from corneal stromal cell cultures was reverse-transcribed and then amplified by the polymerase chain reaction (PCR) using defined, arbitrary primers. The products were displayed on polyacrylamide gels and bands that were differentially expressed were excised, re-amplified and subcloned. The resulting clones were sequenced and utilized as probes for Northern blots with cultured cell RNA or Southern blots of corneal cDNA. One of the products that appeared to be more highly expressed in keratoconus cultures and corneas displayed 100% homology with leukocyte common antigen related protein (LAR), a transmembrane phosphotyrosine phosphatase. Western analyses and immunohistochemistry with monoclonal and/or polyclonal antibodies to LAR were used to examine keratocyte cultures and fresh frozen normal, keratoconus and pseudophakic bullous corneas. We identified a gene product with 100% homology to LAR that is expressed at the RNA level in keratoconus corneas and cell cultures but is found only at low or undetectable levels in normal cultures and normal and pseudophakic bullous keratopathy (PBK) corneas. By Western blotting and immunofluorescence with specific LAR antibodies, the protein was identified in keratoconus stromal cell cultures but not in normal cultures. When fresh frozen tissue was examined, LAR protein was localized to numerous stromal cells throughout central keratoconus corneas, while no central staining was seen in normal or bullous keratopathy corneas. LAR, a transmembrane phosphotyrosine phosphatase, is more highly expressed in keratoconus corneas and stromal cell cultures as demonstrated by differential display, Northern analyses, immunohistochemistry and Western blotting." @default.
- W2026695979 created "2016-06-24" @default.
- W2026695979 creator A5011960217 @default.
- W2026695979 creator A5045792511 @default.
- W2026695979 creator A5046726255 @default.
- W2026695979 creator A5058513785 @default.
- W2026695979 creator A5078489850 @default.
- W2026695979 creator A5090570484 @default.
- W2026695979 date "1999-03-01" @default.
- W2026695979 modified "2023-10-10" @default.
- W2026695979 title "Enhanced Expression of a Transmembrane Phosphotyrosine Phosphatase (LAR) in Keratoconus Cultures and Corneas" @default.
- W2026695979 doi "https://doi.org/10.1006/exer.1998.0604" @default.
- W2026695979 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10079136" @default.
- W2026695979 hasPublicationYear "1999" @default.
- W2026695979 type Work @default.
- W2026695979 sameAs 2026695979 @default.
- W2026695979 citedByCount "13" @default.
- W2026695979 countsByYear W20266959792013 @default.
- W2026695979 countsByYear W20266959792022 @default.
- W2026695979 crossrefType "journal-article" @default.
- W2026695979 hasAuthorship W2026695979A5011960217 @default.
- W2026695979 hasAuthorship W2026695979A5045792511 @default.
- W2026695979 hasAuthorship W2026695979A5046726255 @default.
- W2026695979 hasAuthorship W2026695979A5058513785 @default.
- W2026695979 hasAuthorship W2026695979A5078489850 @default.
- W2026695979 hasAuthorship W2026695979A5090570484 @default.
- W2026695979 hasConcept C104317684 @default.
- W2026695979 hasConcept C153911025 @default.
- W2026695979 hasConcept C16930146 @default.
- W2026695979 hasConcept C169760540 @default.
- W2026695979 hasConcept C2776882836 @default.
- W2026695979 hasConcept C2778918178 @default.
- W2026695979 hasConcept C502942594 @default.
- W2026695979 hasConcept C55493867 @default.
- W2026695979 hasConcept C85265743 @default.
- W2026695979 hasConcept C86803240 @default.
- W2026695979 hasConceptScore W2026695979C104317684 @default.
- W2026695979 hasConceptScore W2026695979C153911025 @default.
- W2026695979 hasConceptScore W2026695979C16930146 @default.
- W2026695979 hasConceptScore W2026695979C169760540 @default.
- W2026695979 hasConceptScore W2026695979C2776882836 @default.
- W2026695979 hasConceptScore W2026695979C2778918178 @default.
- W2026695979 hasConceptScore W2026695979C502942594 @default.
- W2026695979 hasConceptScore W2026695979C55493867 @default.
- W2026695979 hasConceptScore W2026695979C85265743 @default.
- W2026695979 hasConceptScore W2026695979C86803240 @default.
- W2026695979 hasIssue "3" @default.
- W2026695979 hasLocation W20266959791 @default.
- W2026695979 hasLocation W20266959792 @default.
- W2026695979 hasOpenAccess W2026695979 @default.
- W2026695979 hasPrimaryLocation W20266959791 @default.
- W2026695979 hasRelatedWork W1593460714 @default.
- W2026695979 hasRelatedWork W1965328213 @default.
- W2026695979 hasRelatedWork W1983160697 @default.
- W2026695979 hasRelatedWork W2064177132 @default.
- W2026695979 hasRelatedWork W2090387971 @default.
- W2026695979 hasRelatedWork W2372383041 @default.
- W2026695979 hasRelatedWork W2410944156 @default.
- W2026695979 hasRelatedWork W2944452296 @default.
- W2026695979 hasRelatedWork W3130064608 @default.
- W2026695979 hasRelatedWork W2317646458 @default.
- W2026695979 hasVolume "68" @default.
- W2026695979 isParatext "false" @default.
- W2026695979 isRetracted "false" @default.
- W2026695979 magId "2026695979" @default.
- W2026695979 workType "article" @default.