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• W2026700862 abstract "A neuraminidase-encoding gene nanH of Bacteroides fragilis strain YCH46 was cloned into the cosmid vector pHC79. The nanH gene was subcloned from the cosmid and was located within a 2.2-kb XhoI-KpnI fragment. Southern hybridization experiments demonstrated that the gene was present as a single copy on the bacterial chromosome. Neuraminidase activity expressed in the initial Escherichia coli clone was approximately 3600-fold lower than that expressed in B. fragilis YCH46. However, when nanH was transferred from E. coli to B. uniformis by mobilization of a shuttle plasmid, the transconjugant expressed 1100-fold higher activity than the E. coli donor did. These results suggest that modes of nanH expression in E. coli and Bacteroides are heterologous." @default.
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• W2026700862 date "1994-08-01" @default.
• W2026700862 modified "2023-09-24" @default.
• W2026700862 title "Cloning and expression of theBacteroides fragilisYCH46 neuraminidase gene inEschirichia coliandBacteroides uniformis" @default.
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• W2026700862 doi "https://doi.org/10.1111/j.1574-6968.1994.tb07092.x" @default.
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