FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026756048> ?p ?o ?g. }

• W2026756048 endingPage "1101" @default.
• W2026756048 startingPage "1094" @default.
• W2026756048 abstract "Reconstitution of factor VIII from isolated heavy chain (HC) and light chain (LC) shows pH-dependence. In the presence of Ca2+, up to 80% of native factor VIII activity was recovered over a wide range of pH. In contrast, affinity of HC and LC was maximal at pH 6.5-6.75 (Kd approximately 4 nM), whereas a Kd approximately 20 nM was observed at physiological pH (7.25). The effect of Cu2+ (0.5 microM total Cu2+) on maximal activity regenerated was negligible at pH 6.25-8.0. However, this level of Cu2+ increased the inter-chain affinity by approximately 5-fold at pH 7.25. This effect resulted from an approximately 1.5-fold increased association rate constant (k(on)) and an approximately 3-fold reduced dissociation rate constant (k(off)). High affinity (Kd=5.3 fM) of the factor VIII heterodimer for Cu2+ was estimated by increases in cofactor activity. No significant increase in inter-chain affinity was observed when either isolated chain was reacted with Cu2+ followed by addition of the complementary chain. Together, these results suggest that the protonation state of specific residues modulates inter-chain affinity. Furthermore, copper ion contributes to the maintenance of the heterodimer at physiologic pH by a mechanism consistent with bridging the two chains." @default.
• W2026756048 created "2016-06-24" @default.
• W2026756048 creator A5004695750 @default.
• W2026756048 creator A5008757028 @default.
• W2026756048 creator A5010903860 @default.
• W2026756048 creator A5047173459 @default.
• W2026756048 creator A5054167197 @default.
• W2026756048 creator A5068615364 @default.
• W2026756048 creator A5084123532 @default.
• W2026756048 date "2006-06-01" @default.
• W2026756048 modified "2023-10-02" @default.
• W2026756048 title "pH-dependent association of factor VIII chains: Enhancement of affinity at physiological pH by Cu2+" @default.
• W2026756048 cites W1239656452 @default.
• W2026756048 cites W1427181771 @default.
• W2026756048 cites W1525441378 @default.
• W2026756048 cites W1527081709 @default.
• W2026756048 cites W1539899414 @default.
• W2026756048 cites W1570269678 @default.
• W2026756048 cites W1577488314 @default.
• W2026756048 cites W1598297039 @default.
• W2026756048 cites W1622292584 @default.
• W2026756048 cites W1965777297 @default.
• W2026756048 cites W1967104104 @default.
• W2026756048 cites W1976406172 @default.
• W2026756048 cites W1985887127 @default.
• W2026756048 cites W1986705405 @default.
• W2026756048 cites W198936754 @default.
• W2026756048 cites W2000894735 @default.
• W2026756048 cites W2005058131 @default.
• W2026756048 cites W2009004064 @default.
• W2026756048 cites W2010375990 @default.
• W2026756048 cites W2012128918 @default.
• W2026756048 cites W2016657349 @default.
• W2026756048 cites W2019227949 @default.
• W2026756048 cites W2021209687 @default.
• W2026756048 cites W2021283345 @default.
• W2026756048 cites W2036160555 @default.
• W2026756048 cites W2038270465 @default.
• W2026756048 cites W2065336178 @default.
• W2026756048 cites W2067862142 @default.
• W2026756048 cites W2075746234 @default.
• W2026756048 cites W2109711122 @default.
• W2026756048 cites W2128635872 @default.
• W2026756048 cites W2276193850 @default.
• W2026756048 cites W2281427661 @default.
• W2026756048 cites W4293247451 @default.
• W2026756048 doi "https://doi.org/10.1016/j.bbapap.2006.04.004" @default.
• W2026756048 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3731048" @default.
• W2026756048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16731058" @default.
• W2026756048 hasPublicationYear "2006" @default.
• W2026756048 type Work @default.
• W2026756048 sameAs 2026756048 @default.
• W2026756048 citedByCount "11" @default.
• W2026756048 countsByYear W20267560482013 @default.
• W2026756048 countsByYear W20267560482019 @default.
• W2026756048 crossrefType "journal-article" @default.
• W2026756048 hasAuthorship W2026756048A5004695750 @default.
• W2026756048 hasAuthorship W2026756048A5008757028 @default.
• W2026756048 hasAuthorship W2026756048A5010903860 @default.
• W2026756048 hasAuthorship W2026756048A5047173459 @default.
• W2026756048 hasAuthorship W2026756048A5054167197 @default.
• W2026756048 hasAuthorship W2026756048A5068615364 @default.
• W2026756048 hasAuthorship W2026756048A5084123532 @default.
• W2026756048 hasBestOaLocation W20267560482 @default.
• W2026756048 hasConcept C102931765 @default.
• W2026756048 hasConcept C12554922 @default.
• W2026756048 hasConcept C145148216 @default.
• W2026756048 hasConcept C170493617 @default.
• W2026756048 hasConcept C178790620 @default.
• W2026756048 hasConcept C181199279 @default.
• W2026756048 hasConcept C185592680 @default.
• W2026756048 hasConcept C197957613 @default.
• W2026756048 hasConcept C30095370 @default.
• W2026756048 hasConcept C55493867 @default.
• W2026756048 hasConcept C71240020 @default.
• W2026756048 hasConcept C74998103 @default.
• W2026756048 hasConcept C8010536 @default.
• W2026756048 hasConcept C86803240 @default.
• W2026756048 hasConceptScore W2026756048C102931765 @default.
• W2026756048 hasConceptScore W2026756048C12554922 @default.
• W2026756048 hasConceptScore W2026756048C145148216 @default.
• W2026756048 hasConceptScore W2026756048C170493617 @default.
• W2026756048 hasConceptScore W2026756048C178790620 @default.
• W2026756048 hasConceptScore W2026756048C181199279 @default.
• W2026756048 hasConceptScore W2026756048C185592680 @default.
• W2026756048 hasConceptScore W2026756048C197957613 @default.
• W2026756048 hasConceptScore W2026756048C30095370 @default.
• W2026756048 hasConceptScore W2026756048C55493867 @default.
• W2026756048 hasConceptScore W2026756048C71240020 @default.
• W2026756048 hasConceptScore W2026756048C74998103 @default.
• W2026756048 hasConceptScore W2026756048C8010536 @default.
• W2026756048 hasConceptScore W2026756048C86803240 @default.
• W2026756048 hasIssue "6" @default.
• W2026756048 hasLocation W20267560481 @default.
• W2026756048 hasLocation W20267560482 @default.
• W2026756048 hasLocation W20267560483 @default.
• W2026756048 hasLocation W20267560484 @default.
• W2026756048 hasOpenAccess W2026756048 @default.

• next