Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026792004> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2026792004 endingPage "54" @default.
- W2026792004 startingPage "47" @default.
- W2026792004 abstract "To evaluate the antigenicity of a peptide representing a part of the second extracellular loop of the human muscarinic acetylcholine receptor 3 (m3AChR) with autoimmune sera from primary Sjogren's syndrome (pSS), enzyme-linked immunosorbent assays (ELISAs) were developed. On the basis of the computer-predicted data, a 16-mer synthetic peptide KRTVPPGECFIQFLSE (KRSE213-228) was produced by solid-phase peptide synthesis. cDNA coding for the KRSE peptide was chemically synthetized and utilized to express the recombinant glutathione S-transferase (GST)-KRSE fusion protein. The immunoreactivities of the two antigens were tested in ELISAs with the sera of 40 pSS patients and 40 healthy controls. The specificity of the reaction was confirmed by inhibition assays and immunoblottings. The pSS sera resulted in significantly higher mean optical densities than those of the healthy controls (KRSE: 0.4149 vs 0.1494, p<0.0001; GST-KRSE 0.4765 vs 0.1764, p<0.0001). The immunological recognition with the recombinant fusion antigen was significantly better than that for the free peptide (p=0.0068). The sensitivities of the assays were 77.5% (KRSE) and 97% (GST-KRSE). The results of the concentration-dependent inhibition assays by the two systems of peptide presentation indicated that the KRSE sequence is specific for pSS sera. This is the first demonstration of the antigenicity of a novel peptide fragment of the human m3AChR in pSS. The analysed peptide could be of diagnostic relevance." @default.
- W2026792004 created "2016-06-24" @default.
- W2026792004 creator A5004120323 @default.
- W2026792004 creator A5011523467 @default.
- W2026792004 creator A5062258994 @default.
- W2026792004 creator A5079688975 @default.
- W2026792004 creator A5080791321 @default.
- W2026792004 creator A5086573810 @default.
- W2026792004 creator A5091539764 @default.
- W2026792004 date "2005-02-01" @default.
- W2026792004 modified "2023-10-18" @default.
- W2026792004 title "A peptide of human muscarinic acetylcholine receptor 3 is antigenic in primary Sjögren's syndrome" @default.
- W2026792004 cites W1726911736 @default.
- W2026792004 cites W1972637161 @default.
- W2026792004 cites W1975085160 @default.
- W2026792004 cites W1994394606 @default.
- W2026792004 cites W2016870642 @default.
- W2026792004 cites W2022366725 @default.
- W2026792004 cites W2035807387 @default.
- W2026792004 cites W2037647765 @default.
- W2026792004 cites W2061268262 @default.
- W2026792004 cites W2111228958 @default.
- W2026792004 cites W2167385753 @default.
- W2026792004 cites W2320556745 @default.
- W2026792004 doi "https://doi.org/10.1016/j.jaut.2004.11.005" @default.
- W2026792004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15725576" @default.
- W2026792004 hasPublicationYear "2005" @default.
- W2026792004 type Work @default.
- W2026792004 sameAs 2026792004 @default.
- W2026792004 citedByCount "34" @default.
- W2026792004 countsByYear W20267920042012 @default.
- W2026792004 countsByYear W20267920042013 @default.
- W2026792004 countsByYear W20267920042014 @default.
- W2026792004 countsByYear W20267920042015 @default.
- W2026792004 countsByYear W20267920042016 @default.
- W2026792004 countsByYear W20267920042023 @default.
- W2026792004 crossrefType "journal-article" @default.
- W2026792004 hasAuthorship W2026792004A5004120323 @default.
- W2026792004 hasAuthorship W2026792004A5011523467 @default.
- W2026792004 hasAuthorship W2026792004A5062258994 @default.
- W2026792004 hasAuthorship W2026792004A5079688975 @default.
- W2026792004 hasAuthorship W2026792004A5080791321 @default.
- W2026792004 hasAuthorship W2026792004A5086573810 @default.
- W2026792004 hasAuthorship W2026792004A5091539764 @default.
- W2026792004 hasConcept C104317684 @default.
- W2026792004 hasConcept C116289061 @default.
- W2026792004 hasConcept C123894998 @default.
- W2026792004 hasConcept C147483822 @default.
- W2026792004 hasConcept C153911025 @default.
- W2026792004 hasConcept C170493617 @default.
- W2026792004 hasConcept C185592680 @default.
- W2026792004 hasConcept C195616568 @default.
- W2026792004 hasConcept C203014093 @default.
- W2026792004 hasConcept C2779026604 @default.
- W2026792004 hasConcept C2779281246 @default.
- W2026792004 hasConcept C33789571 @default.
- W2026792004 hasConcept C40767141 @default.
- W2026792004 hasConcept C55493867 @default.
- W2026792004 hasConcept C80161118 @default.
- W2026792004 hasConcept C86803240 @default.
- W2026792004 hasConceptScore W2026792004C104317684 @default.
- W2026792004 hasConceptScore W2026792004C116289061 @default.
- W2026792004 hasConceptScore W2026792004C123894998 @default.
- W2026792004 hasConceptScore W2026792004C147483822 @default.
- W2026792004 hasConceptScore W2026792004C153911025 @default.
- W2026792004 hasConceptScore W2026792004C170493617 @default.
- W2026792004 hasConceptScore W2026792004C185592680 @default.
- W2026792004 hasConceptScore W2026792004C195616568 @default.
- W2026792004 hasConceptScore W2026792004C203014093 @default.
- W2026792004 hasConceptScore W2026792004C2779026604 @default.
- W2026792004 hasConceptScore W2026792004C2779281246 @default.
- W2026792004 hasConceptScore W2026792004C33789571 @default.
- W2026792004 hasConceptScore W2026792004C40767141 @default.
- W2026792004 hasConceptScore W2026792004C55493867 @default.
- W2026792004 hasConceptScore W2026792004C80161118 @default.
- W2026792004 hasConceptScore W2026792004C86803240 @default.
- W2026792004 hasIssue "1" @default.
- W2026792004 hasLocation W20267920041 @default.
- W2026792004 hasLocation W20267920042 @default.
- W2026792004 hasOpenAccess W2026792004 @default.
- W2026792004 hasPrimaryLocation W20267920041 @default.
- W2026792004 hasRelatedWork W2013855543 @default.
- W2026792004 hasRelatedWork W2351331285 @default.
- W2026792004 hasRelatedWork W2353117053 @default.
- W2026792004 hasRelatedWork W2353441596 @default.
- W2026792004 hasRelatedWork W2354450666 @default.
- W2026792004 hasRelatedWork W2362309714 @default.
- W2026792004 hasRelatedWork W2371993564 @default.
- W2026792004 hasRelatedWork W2379291194 @default.
- W2026792004 hasRelatedWork W2393364397 @default.
- W2026792004 hasRelatedWork W2429425663 @default.
- W2026792004 hasVolume "24" @default.
- W2026792004 isParatext "false" @default.
- W2026792004 isRetracted "false" @default.
- W2026792004 magId "2026792004" @default.
- W2026792004 workType "article" @default.