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- W2026798424 abstract "Marine nitrifying bacteria have been isolated from surface waters collected in the north Pacific Ocean and off the pier of the Scripps Institution of Oceanography. Two isolates, one ammonium-oxidizer and one nitrite-oxidizer, were freed from heterotrophic contaminants by a dilution technique. Nitrosocystis oceanus was purified by picking cells off an agar surface. Optimal temperature for nitrification in sea water was 28° C. Substrate was oxidized after lag or induction periods of various durations at 37, 20, and, with some cultures, at 12°C. Q10 values varied between 1.3 and 3.0.Growth rates of the nitrifiers increased with increasing substrate concentrations over the range 1–5000μg-at N/l but the increase was not great (ca 10-fold), especially at 18°C. The growth constant, at 18°C, for ammonium oxidizers varied between 0.03 and 1.0 day−1 with ammonium ion concentrations of between 1.5 and 5000 μg-at NH4+-N/l. With some nitrifiers there were lags of up to 50 days before substrate oxidation commenced. All nitrifiers could oxidize substrates when these were present in ecologically significant concentrations.It is possible to predict from these and other studies the time necessary to produce a certain amount of nitrite or nitrate in situ. Predicted rates of productions are too high and unless nitrifiers and substrates are concentrated considerably in micro-zones in the sea it seems likely that other organisms and mechanisms of nitrification are operative." @default.
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- W2026798424 date "1968-07-01" @default.
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- W2026798424 title "The isolation, purification and some kinetic studies of marine nitrifying bacteria" @default.
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- W2026798424 doi "https://doi.org/10.1016/0022-0981(68)90006-3" @default.
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