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• W2026843245 abstract "32P-labeled virus-specific 42- and 26-S RNA has been prepared from BHK 21 cells infected with the togavirus Semliki Forest virus. Analyses of the oligonucleotides generated from these RNA species after digestion with RNAse T1 by two-dimensional polyacrylamide gel electrophoresis (T1-fingerprints) show that both RNA species contain poly(A) sequences and that the oligonucleotides of the 26-S RNA represent a subset of those present in 42-S RNA. The following findings indicate that the different nucleotide sequences are present on the 42-S RNA in the order 5′-terminus-42-S RNA-specific sequences-26-S RNA sequences-poly(A)-AOH. (1) Identical oligonucleotide patterns are found in the T1-fingerprints of the 26-S RNA accumulating in infected cells and of poly(A)-containing fragments of the 42-S RNA which sediment around 26 S on sucrose density gradients. (2) The poly(A) fragments were isolated from T1-fingerprints of 42-and 26-S RNA, digested with pancreatic RNAse, and the reaction products analyzed by electrophoresis on DEAE paper. The results indicate that both fragments contain a heteropolymeric sequence consisting of U6C2(AC)1 (AU)1(AAU)1. (3) GMP has been detected in all oligonucleotides derived from T1-fingerprints which have been analyzed so far, except in the poly(A)-containing oligonucleotides, indicating that the latter contain the 3′-termini of the molecules from which they were derived. (4) [3H]Adenosine-labeled 42-S RNA was digested with RNAse T1 and pancreatic RNAse, the poly(A) fragments were isolated, and an [3H]adenosine to [3H]AMP radioactivity ratio of 1 to 97 was determined for these fragments. Possible implications of the organization of the different sequences on the 42-S RNA on its translation and the replication of SFV are discussed." @default.
• W2026843245 created "2016-06-24" @default.
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• W2026843245 date "1976-08-01" @default.
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• W2026843245 title "Localization of the 26-S RNA sequence on the viral genome type 42-S RNA isolated from SFV-infected cells" @default.
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• W2026843245 doi "https://doi.org/10.1016/0042-6822(76)90073-8" @default.
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