Matches in SemOpenAlex for { <https://semopenalex.org/work/W2026856836> ?p ?o ?g. }
- W2026856836 endingPage "224" @default.
- W2026856836 startingPage "217" @default.
- W2026856836 abstract "The vestibular labyrinth is the organ for sensation of equilibrium. It is part of the inner ear and located in the caudodorsal aspect of the temporal bone which makes it very difficult to access. This study evaluated a preparation technique in cats and dogs for morphological and DNA analysis. The study included 44 temporal bones of 14 cats and 11 dogs collected within 48h after death. Preparation was performed after peri-/endolymphatic injection of Fast-Green-FCF through the fenestra vestibuli to visualize the membranous labyrinth. The vestibular nerve, including its ganglion, and the vestibular labyrinth were exposed by drilling and cracking of the petrous temporal bone along the meatus acusticus internus. The posterior ampulla was collected for histology and transmission electron microscopy whereas the vestibular nerve, the utriculus, sacculus, and the lateral and anterior ampullae were harvested for subsequent DNA analysis. Histology and electron microscopy showed well-preserved cells. A total DNA amount of 4753+/-1502ng in cats and 5865+/-2911ng in dogs was retrieved from the ganglion, and 2390+/-561ng in cats and 2544+/-1277ng in dogs, respectively, from membranous vestibular organs. Polymerase chain reaction of a 229 base pair product of the Gapdh-gene proved for presence of amplifiable DNA. Taken together, mechanical bone removal after Fast-Green-FCF injection allows for reliable gross, microscopic and ultrastructural examination of the feline and canine vestibular labyrinth, and it does not interfere with DNA analysis via PCR. This technique is feasible for multimodal investigation of the vestibular labyrinth retrieved from individual necropsy cases." @default.
- W2026856836 created "2016-06-24" @default.
- W2026856836 creator A5004775967 @default.
- W2026856836 creator A5030015197 @default.
- W2026856836 creator A5031223319 @default.
- W2026856836 creator A5046844736 @default.
- W2026856836 creator A5052864648 @default.
- W2026856836 date "2009-02-01" @default.
- W2026856836 modified "2023-10-02" @default.
- W2026856836 title "A rapid approach to ultrastructural evaluation and DNA analysis of the vestibular labyrinth and ganglion in dogs and cats" @default.
- W2026856836 cites W1963870662 @default.
- W2026856836 cites W1968348656 @default.
- W2026856836 cites W1976678797 @default.
- W2026856836 cites W1976857864 @default.
- W2026856836 cites W1976924356 @default.
- W2026856836 cites W1982877642 @default.
- W2026856836 cites W1984072234 @default.
- W2026856836 cites W1984791625 @default.
- W2026856836 cites W1996686864 @default.
- W2026856836 cites W2008267335 @default.
- W2026856836 cites W2013640200 @default.
- W2026856836 cites W2020265153 @default.
- W2026856836 cites W2028456959 @default.
- W2026856836 cites W2030383656 @default.
- W2026856836 cites W2031903526 @default.
- W2026856836 cites W2035495803 @default.
- W2026856836 cites W2039026849 @default.
- W2026856836 cites W2042570292 @default.
- W2026856836 cites W2049156910 @default.
- W2026856836 cites W2054013172 @default.
- W2026856836 cites W2054026808 @default.
- W2026856836 cites W2062919317 @default.
- W2026856836 cites W2068062158 @default.
- W2026856836 cites W2084327744 @default.
- W2026856836 cites W2086161655 @default.
- W2026856836 cites W2088201501 @default.
- W2026856836 cites W2094663077 @default.
- W2026856836 cites W2096751340 @default.
- W2026856836 cites W2115966151 @default.
- W2026856836 cites W2142594224 @default.
- W2026856836 cites W2148344563 @default.
- W2026856836 cites W2148627945 @default.
- W2026856836 cites W2162144136 @default.
- W2026856836 cites W2165309322 @default.
- W2026856836 cites W2329343706 @default.
- W2026856836 doi "https://doi.org/10.1016/j.jneumeth.2008.10.003" @default.
- W2026856836 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18984005" @default.
- W2026856836 hasPublicationYear "2009" @default.
- W2026856836 type Work @default.
- W2026856836 sameAs 2026856836 @default.
- W2026856836 citedByCount "5" @default.
- W2026856836 countsByYear W20268568362014 @default.
- W2026856836 countsByYear W20268568362016 @default.
- W2026856836 crossrefType "journal-article" @default.
- W2026856836 hasAuthorship W2026856836A5004775967 @default.
- W2026856836 hasAuthorship W2026856836A5030015197 @default.
- W2026856836 hasAuthorship W2026856836A5031223319 @default.
- W2026856836 hasAuthorship W2026856836A5046844736 @default.
- W2026856836 hasAuthorship W2026856836A5052864648 @default.
- W2026856836 hasConcept C105702510 @default.
- W2026856836 hasConcept C126322002 @default.
- W2026856836 hasConcept C142724271 @default.
- W2026856836 hasConcept C162903589 @default.
- W2026856836 hasConcept C169760540 @default.
- W2026856836 hasConcept C190041318 @default.
- W2026856836 hasConcept C2777340575 @default.
- W2026856836 hasConcept C2777522975 @default.
- W2026856836 hasConcept C2777939775 @default.
- W2026856836 hasConcept C2778500370 @default.
- W2026856836 hasConcept C2779152698 @default.
- W2026856836 hasConcept C2779868778 @default.
- W2026856836 hasConcept C2779904517 @default.
- W2026856836 hasConcept C2781334511 @default.
- W2026856836 hasConcept C2910076437 @default.
- W2026856836 hasConcept C71924100 @default.
- W2026856836 hasConcept C86803240 @default.
- W2026856836 hasConceptScore W2026856836C105702510 @default.
- W2026856836 hasConceptScore W2026856836C126322002 @default.
- W2026856836 hasConceptScore W2026856836C142724271 @default.
- W2026856836 hasConceptScore W2026856836C162903589 @default.
- W2026856836 hasConceptScore W2026856836C169760540 @default.
- W2026856836 hasConceptScore W2026856836C190041318 @default.
- W2026856836 hasConceptScore W2026856836C2777340575 @default.
- W2026856836 hasConceptScore W2026856836C2777522975 @default.
- W2026856836 hasConceptScore W2026856836C2777939775 @default.
- W2026856836 hasConceptScore W2026856836C2778500370 @default.
- W2026856836 hasConceptScore W2026856836C2779152698 @default.
- W2026856836 hasConceptScore W2026856836C2779868778 @default.
- W2026856836 hasConceptScore W2026856836C2779904517 @default.
- W2026856836 hasConceptScore W2026856836C2781334511 @default.
- W2026856836 hasConceptScore W2026856836C2910076437 @default.
- W2026856836 hasConceptScore W2026856836C71924100 @default.
- W2026856836 hasConceptScore W2026856836C86803240 @default.
- W2026856836 hasIssue "1" @default.
- W2026856836 hasLocation W20268568361 @default.
- W2026856836 hasLocation W20268568362 @default.
- W2026856836 hasOpenAccess W2026856836 @default.
- W2026856836 hasPrimaryLocation W20268568361 @default.