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- W2026875627 abstract "Dextransucrase was produced from a Leuconostoc mesenteroides isolated from pulque, a traditional Aztec alcoholic beverage produced from agave juice containing sucrose as the main carbon source. Almost all the dextransucrase activity (87%) was associated with the cells, and was unusually high (1.04 U mg−1 of cells). The culture medium composition was optimized through a Box-Behnken method resulting in a process yielding 2.2 U ml−1 of insoluble glucosyltransferase activity. The enzyme had a molecular weight of 166 kDa. Optimal temperature was 35°C with a half-life of 137 min at the same temperature. As with dextransucrase from the industrial strain L. mesenteroides NRRL B-512F, the enzyme showed Michaelis–Menten kinetic behavior with excess substrate inhibition (Km and Ki values of 0.026 M and 1.23 M respectively); produced soluble linear dextran with glucose molecules linked mainly in α(1–6) with branching in α(1–3) in a proportion of 4:1 as shown by NMR studies; and produced a high yield of isomalto-oligosaccharides in the presence of maltose." @default.
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- W2026875627 date "1998-08-01" @default.
- W2026875627 modified "2023-10-14" @default.
- W2026875627 title "Production and properties of a dextransucrase from Leuconostoc mesenteroides IBT-PQ isolated from ' pulque ', a traditional Aztec alcoholic beverage" @default.
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- W2026875627 doi "https://doi.org/10.1038/sj.jim.2900560" @default.
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