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- W2026908887 abstract "With the availability of the complete yeast genomic sequence, techniques which allow the rapid functional analysis of genes of interest are of increasing importance. Here we report a technique which allows the initial characterisation of genes of interest, through the construction of conditionally expressed mutations for functional analyses and the generation of epitope-tagged fusion proteins for immuno-localisation and immuno-purification, entirely by PCR." @default.
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- W2026908887 title "One-step PCR mediated strategy for the construction of conditionally expressed and epitope tagged yeast proteins" @default.
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- W2026908887 doi "https://doi.org/10.1093/nar/24.17.3469" @default.
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