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- W2026932374 abstract "A hybridoma (F4B) which produced a monoclonal antibody (mAb) specific for serotype g carbohydrate antigen (RRg) of Streptococcus mutans 6715 was obtained. The F4B mAb cross-reacted with purified carbohydrate antigens of serotype d (RRd) and serotype h (TCAh). In immunodiffusion tests, F4B mAb produced a stable precipitin band with RRg, while the band developed between the mAb and RRd/TChh in the cold disappeared when incubated at room temperature. The immunoprecipitin reaction between F4B mAb and RRg was strongly inhibited upon addition of lactose. Unter Verwendung von BALB/c Sp2/0-Ag 14 Myelomzellen und Milzzellen von Mäusen, die mit Streptococcus mutans 6715 (Serotyp g) immunisiert waren, wurde eine Hybridomlinie (F4B) gewonnen, die einen monoklonalen Antikörper (mAb) gegen das typenspezifische Polysaccharidantigen (RRg) bildete. Der mAb erwies sich als IgM (μ. und η) und führte zu Kreuzreaktionen mit gereinigten Kohlenhydratantigenen der S. mutans Sero typen d (RRd) und h (TCAh). Im Immundiffusionstest bildete der F4B mAb eine stabile Präzipitationsbande mit RRg, wogegen sich die bei 4°C gebildeten Banden zwischen dem mAb und RRd/TCAh bei weiterer Inkubation bei 25°C wieder auflösten. Die Präzipitationsreaktion zwischen dem F4B mAb und RRg wird zu 40 % durch Laktose inhibiert." @default.
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- W2026932374 date "1985-04-01" @default.
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- W2026932374 title "Antigen analyses of serotypes of streptococcus mutans using a monoclonal antibody elaborated against serotype g polysaccharide antigen" @default.
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- W2026932374 doi "https://doi.org/10.1016/s0176-6724(85)80055-9" @default.
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