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• W2026974566 abstract "1 We employed the perforated patch whole-cell technique to investigate the effects of ATP and other related nucleotides on membrane conductances in avian exocrine salt gland cells. 2 ATP (10μm-1 mM) evoked an increase in maxi-K+ and Cl− conductances with a reversal potential of −35mV. At lower concentrations of ATP (≤100 μm) responses were generally oscillatory with a sustained response observed at higher concentrations (≥200 μm). 3 Both oscillatory and sustained responses were abolished by the removal of bath Ca2+. In cells preincubated in extracellular saline containing reduced Ca2+, the application of ATP resulted in a transient increase in current. 4 As increasing concentrations of ATP (and related nucleotides) evoked a graded sequence of events with little run-down we were able to establish a rank order of potency in single cells. The order of potency of ATP analogues and agonists of the various P2-receptor subtypes was UTP>ATP = 2-methylthio-ATP>ADP. Adenosine (1μm-1mm), AMP (1μm-1μm), α,β-methylene-ATP (1 μM−1 mm) and α,β-methylene-ATP (1μm- mm) were without effect. 5 In conclusion, although unable to preclude a role for a P2Y-receptor, our results suggest that ATP binds to a P2U-receptor increasing [Ca2+]i and subsequently activating Ca2+-sensitive K+ and Cl−currents." @default.
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• W2026974566 date "1995-05-01" @default.
• W2026974566 modified "2023-09-24" @default.
• W2026974566 title "Activation by ATP of a P2U‘nucleotide’ receptor in an exocrine cell" @default.
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• W2026974566 doi "https://doi.org/10.1111/j.1476-5381.1995.tb15880.x" @default.
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