FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027032015> ?p ?o ?g. }

• W2027032015 endingPage "1587" @default.
• W2027032015 startingPage "1578" @default.
• W2027032015 abstract "The archaeal exosome is a protein complex involved in the degradation and the posttranscriptional tailing of RNA. The proteins Rrp41, Rrp42, Rrp4, Csl4 and DnaG are major subunits of the exosome in Sulfolobus solfataricus. In vitro, Rrp41 and Rrp42 form a catalytically active hexamer, to which an RNA-binding cap of Rrp4 and/or Csl4 is attached. Rrp4 confers strong poly(A) specificity to the exosome. The majority of Rrp41 and DnaG is detectable in the insoluble fraction and is localized at the cell periphery. The aim of this study was to analyze whether there are differences in the composition of the soluble and the insoluble exosomes. We found that the soluble exosome contains less DnaG and less Csl4 than the insoluble exosome which co-sediments with ribosomal subunits in sucrose density gradients. EF1-alpha was co-precipitated with the soluble exosome from S100 fractions using DnaG-directed antibodies, and from density gradient fractions using Rrp41-specific antibodies, strongly suggesting that EF1-alpha is an interaction partner of the soluble exosome. Furthermore, Csl4 was co-immunoprecipitated with the exosome using Rrp4-specific antibodies and vice versa, demonstrating the presence of heteromeric RNA-binding caps in vivo. To address the mechanism for poly(A) recognition by Rrp4, an exosome with an RNA-binding cap composed of truncated Rrp4 lacking the KH domain was reconstituted and analyzed. Although the deletion of the KH domain negatively influenced the degradation activity of the exosome, the poly(A) specificity was retained, showing that the KH domain is dispensable for the strong poly(A) preference of Rrp4." @default.
• W2027032015 created "2016-06-24" @default.
• W2027032015 creator A5010655192 @default.
• W2027032015 creator A5048658410 @default.
• W2027032015 creator A5071709263 @default.
• W2027032015 creator A5076640513 @default.
• W2027032015 creator A5077530012 @default.
• W2027032015 date "2012-07-01" @default.
• W2027032015 modified "2023-10-07" @default.
• W2027032015 title "Heterogeneous complexes of the RNA exosome in Sulfolobus solfataricus" @default.
• W2027032015 cites W1575095861 @default.
• W2027032015 cites W1653560622 @default.
• W2027032015 cites W1965007012 @default.
• W2027032015 cites W1969666715 @default.
• W2027032015 cites W1970208766 @default.
• W2027032015 cites W1975324285 @default.
• W2027032015 cites W1981930726 @default.
• W2027032015 cites W1988309554 @default.
• W2027032015 cites W1989579432 @default.
• W2027032015 cites W1995481648 @default.
• W2027032015 cites W1995557066 @default.
• W2027032015 cites W1999815828 @default.
• W2027032015 cites W2002032465 @default.
• W2027032015 cites W2016247874 @default.
• W2027032015 cites W2016556734 @default.
• W2027032015 cites W2024735335 @default.
• W2027032015 cites W2029021521 @default.
• W2027032015 cites W2032501207 @default.
• W2027032015 cites W2033773344 @default.
• W2027032015 cites W2036634780 @default.
• W2027032015 cites W2036813822 @default.
• W2027032015 cites W2044386270 @default.
• W2027032015 cites W2046593260 @default.
• W2027032015 cites W2048557198 @default.
• W2027032015 cites W2056586488 @default.
• W2027032015 cites W2065636891 @default.
• W2027032015 cites W2068295663 @default.
• W2027032015 cites W207743832 @default.
• W2027032015 cites W2095976136 @default.
• W2027032015 cites W2098152091 @default.
• W2027032015 cites W2098386120 @default.
• W2027032015 cites W2098649870 @default.
• W2027032015 cites W2104030280 @default.
• W2027032015 cites W2107250499 @default.
• W2027032015 cites W2110612352 @default.
• W2027032015 cites W2111809568 @default.
• W2027032015 cites W2122822910 @default.
• W2027032015 cites W2128951244 @default.
• W2027032015 cites W2129232563 @default.
• W2027032015 cites W2136111794 @default.
• W2027032015 cites W2136934911 @default.
• W2027032015 cites W2144414956 @default.
• W2027032015 cites W2145147543 @default.
• W2027032015 cites W2148699435 @default.
• W2027032015 cites W2150176217 @default.
• W2027032015 cites W2163175097 @default.
• W2027032015 cites W68264092 @default.
• W2027032015 doi "https://doi.org/10.1016/j.biochi.2012.03.026" @default.
• W2027032015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22503705" @default.
• W2027032015 hasPublicationYear "2012" @default.
• W2027032015 type Work @default.
• W2027032015 sameAs 2027032015 @default.
• W2027032015 citedByCount "26" @default.
• W2027032015 countsByYear W20270320152012 @default.
• W2027032015 countsByYear W20270320152013 @default.
• W2027032015 countsByYear W20270320152014 @default.
• W2027032015 countsByYear W20270320152017 @default.
• W2027032015 countsByYear W20270320152018 @default.
• W2027032015 countsByYear W20270320152019 @default.
• W2027032015 countsByYear W20270320152020 @default.
• W2027032015 countsByYear W20270320152021 @default.
• W2027032015 countsByYear W20270320152023 @default.
• W2027032015 crossrefType "journal-article" @default.
• W2027032015 hasAuthorship W2027032015A5010655192 @default.
• W2027032015 hasAuthorship W2027032015A5048658410 @default.
• W2027032015 hasAuthorship W2027032015A5071709263 @default.
• W2027032015 hasAuthorship W2027032015A5076640513 @default.
• W2027032015 hasAuthorship W2027032015A5077530012 @default.
• W2027032015 hasConcept C104317684 @default.
• W2027032015 hasConcept C145059251 @default.
• W2027032015 hasConcept C153911025 @default.
• W2027032015 hasConcept C185581394 @default.
• W2027032015 hasConcept C185592680 @default.
• W2027032015 hasConcept C188192080 @default.
• W2027032015 hasConcept C194993378 @default.
• W2027032015 hasConcept C20518536 @default.
• W2027032015 hasConcept C2780631447 @default.
• W2027032015 hasConcept C2781261824 @default.
• W2027032015 hasConcept C550995028 @default.
• W2027032015 hasConcept C552990157 @default.
• W2027032015 hasConcept C55493867 @default.
• W2027032015 hasConcept C58167604 @default.
• W2027032015 hasConcept C67705224 @default.
• W2027032015 hasConcept C75449159 @default.
• W2027032015 hasConcept C86803240 @default.
• W2027032015 hasConceptScore W2027032015C104317684 @default.
• W2027032015 hasConceptScore W2027032015C145059251 @default.
• W2027032015 hasConceptScore W2027032015C153911025 @default.

• next