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• W2027109483 abstract "A semi-automated, immunomagnetic capture-reverse transcription PCR (IMC-RT-PCR) assay for the detection of three pineapple-infecting ampeloviruses, Pineapple mealybug wilt-associated virus-1, -2 and -3, is described. The assay was equivalent in sensitivity but more rapid than conventional immunocapture RT-PCR. The assay can be used either as a one- or two-step RT-PCR and allows detection of the viruses separately or together in a triplex assay from fresh, frozen or freeze-dried pineapple leaf tissue. This IMC-RT-PCR assay could be used for high throughput screening of pineapple planting propagules and could easily be modified for the detection of other RNA viruses in a range of plant species, provided suitable antibodies are available." @default.
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• W2027109483 date "2009-02-01" @default.
• W2027109483 modified "2023-09-25" @default.
• W2027109483 title "Development of an immunomagnetic capture-reverse transcriptase-PCR assay for three pineapple ampeloviruses" @default.
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• W2027109483 doi "https://doi.org/10.1016/j.jviromet.2008.10.006" @default.
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