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• W2027174651 abstract "1. In experiments designed to investigate possible organ- and tumor-specific differences in methylation of soluble RNA reported in the literature, RNA methylase activity from dialyzed high-speed supernatant extracts of adult and newborn mouse organs and from mouse tumors was measured. Soluble RNA from Escherichia coli B was used as acceptor, and tests were made over a range of concentrations of ammonium acetate and/or magnesium chloride. 2. It was found that the rate of methylation by organ and tumor extracts was stimulated 4- to 8-fold, and the extent of methylation 2- to 4-fold by 0.36 M ammonium acetate. 3. The products of methylation in vitro were separated electrophoretically. The pattern of incorporation into nucleotides of C, A, G and U was measured in the presence and absence of additional ions. Addition of ions increased the methylation of each major nucleotide from an average of 2-fold in guanylic acid to almost 9-fold in cytidylic acid. 4. Neither the rate, the extent nor the pattern of methylation served to distinguish unequivocally extracts from tumors or from normal organs, or to characterize extracts of several normal organs." @default.
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• W2027174651 date "1968-04-01" @default.
• W2027174651 modified "2023-10-16" @default.
• W2027174651 title "Methylation of RNA by mouse organs and tumors: Ionic stimulation in vitro" @default.
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• W2027174651 doi "https://doi.org/10.1016/0005-2787(68)90083-x" @default.
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