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• W2027215520 abstract "Herein, a rapid electrochemical biosensor for l-histidine based on highly specific, l-histidine-dependent DNAzymes is described. DNA with a single, sessile ribo-adenine, self-cleaves in the presence of l-histidine, allowing a ferrocene tag to transfer electrons to the electrode. The double-stranded DNA complex was chemi-absorbed to a gold electrode via a 3′ terminal thiol. The signal of this proposed sensor is linear over the range, 1 nM to 10 μM, with R = 0.98775. To improve signal intensity, gold nanoparticles were anchored to a gold electrode surface which had been previously modified with self-assembled monolayers of 1,6-hexanedithiol. With gold nanoparticle modification, a lower detection limit of 0.1 pM l-histidine and a good linear relationship over the range, 0.1 pM to 50 nM were obtained. The proposed biosensor presents high specificity for l-histidine, is not affected by the presence of other amino acids, and demonstrates excellent enantio-selectivity toward l-histidine. This proposed sensor protocol offers reasonable selectivity, rapid speed, and operational convenience for real sample assays." @default.
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• W2027215520 date "2011-01-01" @default.
• W2027215520 modified "2023-09-27" @default.
• W2027215520 title "Electrochemical real-time detection of l-histidine via self-cleavage of DNAzymes" @default.
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• W2027215520 doi "https://doi.org/10.1016/j.bios.2010.10.041" @default.
• W2027215520 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21111599" @default.
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