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- W2027261678 abstract "A method is described for the determination of glutathione reductase activity (GR; EC 1.6.4.2) in plant extracts utilizing HPLC quantitation of NADP+following the reduction of glutathione disulfides. After protein incubation, fluorescence of NADP+was induced under strongly basic conditions, and the product was directly resolved from the reaction medium by isocratic reversed-phase elution on a silica-coated alumina support which took 2 min. The mobile phase was acetonitrile–water (50:50) delivered at a flow rate of 1.5 ml/min. The adduct (stable for at least 7 days) was detected fluorometrically and quantitated by direct integration of peak area." @default.
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- W2027261678 title "High-Performance Liquid Chromatographic Resolution of NADP+after Induction of Fluorescence and Its Application to Assay for an NADPH-Dependent Enzyme: Application to the Determination of Glutathione Reductase Activity in Plant Leaf Extracts" @default.
- W2027261678 doi "https://doi.org/10.1006/abio.1996.0196" @default.
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