FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027280424> ?p ?o ?g. }

• W2027280424 endingPage "274" @default.
• W2027280424 startingPage "265" @default.
• W2027280424 abstract "Polymeric nanoparticles have tremendous potential either as carriers or markers in treatment for diseases or as diagnostics in biomedical applications. Finding the optimal conditions for effective intracellular delivery of the payload to the location of interest is still a big challenge. The particles have to overcome the barrier of the cell membrane. Here, we investigated the uptake in HeLa cells of fluorescent polystyrene particles with different size and surface charge. Particles stabilized with the nonionic surfactant Lutensol AT50® (132 nm, 180 nm, 242 nm, 816 nm, 846 nm diameter) were synthesized via dispersion polymerization. Cationic particles (120 nm, 208 nm, 267 nm, 603 nm diameter) were obtained by a combination of miniemulsion and seed dispersion polymerization using cationic surfactant (cetyltrimethylammonium chloride (CTMA-Cl). The particle uptake into HeLa cells was studied by confocal laser scanning microscopy and flow cytometry. Nonionic particles were – independent of their size – taken up by cells only at a barely detectable level, thus aggravating a quantitative comparison. The uptake of positively charged particles was substantially higher and therefore enabling further investigation keeping constant one of these parameters: either material amount or particles number or total interaction surface area. It was found that the uptake rather depends on the total amount of polymeric material present in the media than on the number of particles. The total particle’s surface area does not correlate linearly with the uptake, thus indicating that there is no direct dependency between the total surface area and the cellular endocytotic process to overcome the biobarrier “cell membrane.” A potentially novel uptake mechanism is found which can be described as an excavator shovel like mechanism. It is a kind of macropinocytosis dependent on actin filaments as well as dynamin, but is clathrin-independent." @default.
• W2027280424 created "2016-06-24" @default.
• W2027280424 creator A5005655542 @default.
• W2027280424 creator A5034838011 @default.
• W2027280424 creator A5050057164 @default.
• W2027280424 creator A5051099606 @default.
• W2027280424 creator A5086488702 @default.
• W2027280424 date "2013-06-01" @default.
• W2027280424 modified "2023-10-17" @default.
• W2027280424 title "Polymeric nanoparticles of different sizes overcome the cell membrane barrier" @default.
• W2027280424 cites W1506654534 @default.
• W2027280424 cites W1603724904 @default.
• W2027280424 cites W1672147349 @default.
• W2027280424 cites W1977052211 @default.
• W2027280424 cites W1983619321 @default.
• W2027280424 cites W1985516113 @default.
• W2027280424 cites W1986821148 @default.
• W2027280424 cites W1988296513 @default.
• W2027280424 cites W1998532938 @default.
• W2027280424 cites W2008410018 @default.
• W2027280424 cites W2025868400 @default.
• W2027280424 cites W2032751507 @default.
• W2027280424 cites W2033960117 @default.
• W2027280424 cites W2034146282 @default.
• W2027280424 cites W2038914834 @default.
• W2027280424 cites W2039123138 @default.
• W2027280424 cites W2040894400 @default.
• W2027280424 cites W2041325852 @default.
• W2027280424 cites W2056673093 @default.
• W2027280424 cites W2072698782 @default.
• W2027280424 cites W2073476855 @default.
• W2027280424 cites W2076107990 @default.
• W2027280424 cites W2076241511 @default.
• W2027280424 cites W2076639767 @default.
• W2027280424 cites W2078057254 @default.
• W2027280424 cites W2083284625 @default.
• W2027280424 cites W2089146874 @default.
• W2027280424 cites W2089716915 @default.
• W2027280424 cites W2095266942 @default.
• W2027280424 cites W2111115146 @default.
• W2027280424 cites W2113972541 @default.
• W2027280424 cites W2117630026 @default.
• W2027280424 cites W2122015259 @default.
• W2027280424 cites W2122167700 @default.
• W2027280424 cites W2124869438 @default.
• W2027280424 cites W2140002597 @default.
• W2027280424 cites W2144101252 @default.
• W2027280424 cites W2154482941 @default.
• W2027280424 cites W2157331626 @default.
• W2027280424 cites W2160473317 @default.
• W2027280424 cites W2161765731 @default.
• W2027280424 cites W2166709102 @default.
• W2027280424 cites W2167806590 @default.
• W2027280424 cites W4253623380 @default.
• W2027280424 doi "https://doi.org/10.1016/j.ejpb.2013.01.024" @default.
• W2027280424 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23422734" @default.
• W2027280424 hasPublicationYear "2013" @default.
• W2027280424 type Work @default.
• W2027280424 sameAs 2027280424 @default.
• W2027280424 citedByCount "57" @default.
• W2027280424 countsByYear W20272804242013 @default.
• W2027280424 countsByYear W20272804242014 @default.
• W2027280424 countsByYear W20272804242015 @default.
• W2027280424 countsByYear W20272804242016 @default.
• W2027280424 countsByYear W20272804242017 @default.
• W2027280424 countsByYear W20272804242018 @default.
• W2027280424 countsByYear W20272804242019 @default.
• W2027280424 countsByYear W20272804242020 @default.
• W2027280424 countsByYear W20272804242021 @default.
• W2027280424 countsByYear W20272804242022 @default.
• W2027280424 countsByYear W20272804242023 @default.
• W2027280424 crossrefType "journal-article" @default.
• W2027280424 hasAuthorship W2027280424A5005655542 @default.
• W2027280424 hasAuthorship W2027280424A5034838011 @default.
• W2027280424 hasAuthorship W2027280424A5050057164 @default.
• W2027280424 hasAuthorship W2027280424A5051099606 @default.
• W2027280424 hasAuthorship W2027280424A5086488702 @default.
• W2027280424 hasConcept C111368507 @default.
• W2027280424 hasConcept C115537861 @default.
• W2027280424 hasConcept C120665830 @default.
• W2027280424 hasConcept C121332964 @default.
• W2027280424 hasConcept C12554922 @default.
• W2027280424 hasConcept C127313418 @default.
• W2027280424 hasConcept C127413603 @default.
• W2027280424 hasConcept C155672457 @default.
• W2027280424 hasConcept C171250308 @default.
• W2027280424 hasConcept C177562468 @default.
• W2027280424 hasConcept C178790620 @default.
• W2027280424 hasConcept C185592680 @default.
• W2027280424 hasConcept C187530423 @default.
• W2027280424 hasConcept C192562407 @default.
• W2027280424 hasConcept C2776024560 @default.
• W2027280424 hasConcept C2777492985 @default.
• W2027280424 hasConcept C2777922577 @default.
• W2027280424 hasConcept C2778517922 @default.
• W2027280424 hasConcept C41625074 @default.
• W2027280424 hasConcept C42360764 @default.
• W2027280424 hasConcept C44228677 @default.

• next