FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027404734> ?p ?o ?g. }

• W2027404734 endingPage "99" @default.
• W2027404734 startingPage "92" @default.
• W2027404734 abstract "The insulin-independent and combined effects of fatty acids (FA; linoleic and oleic acids) and insulin in modulating lipid accumulation and adipogenesis in 3T3-L1 cells was investigated using a novel protocol avoiding the effects of a complex hormone ‘induction’ mixture. 3T3-L1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) plus serum (control) or in DMEM plus either 0.3 mmol/l linoleic or oleic acids with 0.3 mmol/l FA-free bovine serum albumin in the presence or absence of insulin. Cells were cultured for 4 to 8 days and cell number, lipid accumulation, peroxisome proliferator-activated receptor-gamma (PPAR-γ) and glucose transporter 4 (GLUT-4) protein expression were determined. Cell number appeared to be decreased in comparison with control cultures. In both oleic acid and linoleic acid-treated cells, notably in the absence (and presence) of insulin, oil-red O stain-positive cells showed abundant lipid. The percentage of cells showing lipid accumulation was greater in FA-treated cultures compared with control cells grown in DMEM plus serum (P < 0.001). Treatment with both linoleic and oleic acid-containing media evoked higher levels of PPAR-γ than observed in control cultures (P < 0.05). GLUT-4 protein also increased in response to treatment with both linoleic and oleic acid-containing media (P < 0.001). Lipid accumulation in 3T3-L1 cells occurs in response to either oleic or linoleic acids independently of the presence of insulin. Both PPAR-γ and GLUT-4 protein expression were stimulated. Both proteins are considered markers of adipogenesis, and these observations suggest that these cells had entered the physiological state broadly accepted as differentiated. Furthermore, 3T3-L1 cells can be induced to accumulate lipid in a serum-free medium supplemented with FA, without the use of induction protocols using complex hormone mixtures. We have demonstrated a novel model for the study of lipid accumulation that will improve the understanding of adipogenesis in adipocyte lineage cells." @default.
• W2027404734 created "2016-06-24" @default.
• W2027404734 creator A5000428250 @default.
• W2027404734 creator A5043388995 @default.
• W2027404734 creator A5067462511 @default.
• W2027404734 creator A5069104481 @default.
• W2027404734 creator A5074856949 @default.
• W2027404734 creator A5044136445 @default.
• W2027404734 date "2008-01-01" @default.
• W2027404734 modified "2023-09-24" @default.
• W2027404734 title "Regulation of lipid accumulation in 3T3-L1 cells: insulin-independent and combined effects of fatty acids and insulin" @default.
• W2027404734 cites W1547032503 @default.
• W2027404734 cites W1777776805 @default.
• W2027404734 cites W1799473879 @default.
• W2027404734 cites W1934166066 @default.
• W2027404734 cites W1968375269 @default.
• W2027404734 cites W1981101682 @default.
• W2027404734 cites W1984278456 @default.
• W2027404734 cites W1984706102 @default.
• W2027404734 cites W1998329658 @default.
• W2027404734 cites W2002519246 @default.
• W2027404734 cites W2003311380 @default.
• W2027404734 cites W2006259320 @default.
• W2027404734 cites W2016570094 @default.
• W2027404734 cites W2030901818 @default.
• W2027404734 cites W2033580378 @default.
• W2027404734 cites W2035216298 @default.
• W2027404734 cites W2042669405 @default.
• W2027404734 cites W2045358575 @default.
• W2027404734 cites W2050513874 @default.
• W2027404734 cites W2062184039 @default.
• W2027404734 cites W2064112171 @default.
• W2027404734 cites W2073175037 @default.
• W2027404734 cites W2084434190 @default.
• W2027404734 cites W2084763995 @default.
• W2027404734 cites W2093242556 @default.
• W2027404734 cites W2094793382 @default.
• W2027404734 cites W2098243729 @default.
• W2027404734 cites W2102120208 @default.
• W2027404734 cites W2109900934 @default.
• W2027404734 cites W2118397648 @default.
• W2027404734 cites W2118799567 @default.
• W2027404734 cites W2121778053 @default.
• W2027404734 cites W2125344994 @default.
• W2027404734 cites W2138102988 @default.
• W2027404734 cites W2143143724 @default.
• W2027404734 cites W2143808884 @default.
• W2027404734 cites W2146168160 @default.
• W2027404734 cites W2148244700 @default.
• W2027404734 cites W2154433131 @default.
• W2027404734 cites W2164015929 @default.
• W2027404734 cites W2175925218 @default.
• W2027404734 cites W2325110302 @default.
• W2027404734 cites W2407618083 @default.
• W2027404734 cites W4249478443 @default.
• W2027404734 doi "https://doi.org/10.1017/s1751731107000936" @default.
• W2027404734 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22444967" @default.
• W2027404734 hasPublicationYear "2008" @default.
• W2027404734 type Work @default.
• W2027404734 sameAs 2027404734 @default.
• W2027404734 citedByCount "23" @default.
• W2027404734 countsByYear W20274047342012 @default.
• W2027404734 countsByYear W20274047342013 @default.
• W2027404734 countsByYear W20274047342015 @default.
• W2027404734 countsByYear W20274047342016 @default.
• W2027404734 countsByYear W20274047342018 @default.
• W2027404734 countsByYear W20274047342019 @default.
• W2027404734 countsByYear W20274047342021 @default.
• W2027404734 countsByYear W20274047342022 @default.
• W2027404734 countsByYear W20274047342023 @default.
• W2027404734 crossrefType "journal-article" @default.
• W2027404734 hasAuthorship W2027404734A5000428250 @default.
• W2027404734 hasAuthorship W2027404734A5043388995 @default.
• W2027404734 hasAuthorship W2027404734A5044136445 @default.
• W2027404734 hasAuthorship W2027404734A5067462511 @default.
• W2027404734 hasAuthorship W2027404734A5069104481 @default.
• W2027404734 hasAuthorship W2027404734A5074856949 @default.
• W2027404734 hasBestOaLocation W20274047341 @default.
• W2027404734 hasConcept C126322002 @default.
• W2027404734 hasConcept C134018914 @default.
• W2027404734 hasConcept C185592680 @default.
• W2027404734 hasConcept C2779306644 @default.
• W2027404734 hasConcept C2780809830 @default.
• W2027404734 hasConcept C2781403372 @default.
• W2027404734 hasConcept C543025807 @default.
• W2027404734 hasConcept C55493867 @default.
• W2027404734 hasConcept C71924100 @default.
• W2027404734 hasConcept C86803240 @default.
• W2027404734 hasConceptScore W2027404734C126322002 @default.
• W2027404734 hasConceptScore W2027404734C134018914 @default.
• W2027404734 hasConceptScore W2027404734C185592680 @default.
• W2027404734 hasConceptScore W2027404734C2779306644 @default.
• W2027404734 hasConceptScore W2027404734C2780809830 @default.
• W2027404734 hasConceptScore W2027404734C2781403372 @default.
• W2027404734 hasConceptScore W2027404734C543025807 @default.
• W2027404734 hasConceptScore W2027404734C55493867 @default.
• W2027404734 hasConceptScore W2027404734C71924100 @default.
• W2027404734 hasConceptScore W2027404734C86803240 @default.

• next