Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027430031> ?p ?o ?g. }
- W2027430031 endingPage "91" @default.
- W2027430031 startingPage "86" @default.
- W2027430031 abstract "A simple method for the determination of cellular uptake of phytosterols by Caco-2 cells has been developed by ultra performance liquid chromatography with ultraviolet detection (UPLC-UV). UPLC-UV was established using an ODS column, acetonitrile/H(2)O (9:1, v/v) as a mobile phase, and a detection wavelength at 210 nm. As analytes, β-sitosterol, campesterol, stigmasterol, and brassicasterol were selected based on the abundance in foods and the similarity of their structures. A linear relation was observed between the peak area and the amount of sterol injected from 50 to 2000 pmol (r>0.999) with a relative standard deviation (RSD) of less than 2.5% (n=6). This method was applied to the determination of cellular uptake of phytosterols by Caco-2 cells. Recovery tests showed that phytosterols were extracted from the cell lysates by chloroform and determined by UPLC-UV with a recovery rate of more than 80.2% and an RSD of less than 11.3% (n=3). When Caco-2 cells were incubated with phytosterols at 37°C, their uptake was increased with time in a concentration-dependent manner. This method will be useful for the simultaneous determination of cellular phytosterols in an in vitro intestine model." @default.
- W2027430031 created "2016-06-24" @default.
- W2027430031 creator A5027320260 @default.
- W2027430031 creator A5070286775 @default.
- W2027430031 creator A5078945168 @default.
- W2027430031 creator A5082004578 @default.
- W2027430031 date "2012-02-01" @default.
- W2027430031 modified "2023-10-17" @default.
- W2027430031 title "An ultra performance liquid chromatographic method for determining phytosterol uptake by Caco-2 cells" @default.
- W2027430031 cites W1487672542 @default.
- W2027430031 cites W1510167055 @default.
- W2027430031 cites W1860264407 @default.
- W2027430031 cites W1973523583 @default.
- W2027430031 cites W1978203908 @default.
- W2027430031 cites W1982553121 @default.
- W2027430031 cites W1988706034 @default.
- W2027430031 cites W1989872528 @default.
- W2027430031 cites W1996784422 @default.
- W2027430031 cites W1997716632 @default.
- W2027430031 cites W2009633273 @default.
- W2027430031 cites W2024339367 @default.
- W2027430031 cites W2027663543 @default.
- W2027430031 cites W2038681937 @default.
- W2027430031 cites W2039514351 @default.
- W2027430031 cites W2047868393 @default.
- W2027430031 cites W2055609682 @default.
- W2027430031 cites W2059283688 @default.
- W2027430031 cites W2062364166 @default.
- W2027430031 cites W2081706355 @default.
- W2027430031 cites W2082647909 @default.
- W2027430031 cites W2084375116 @default.
- W2027430031 cites W2088983410 @default.
- W2027430031 cites W2094929865 @default.
- W2027430031 cites W2099378835 @default.
- W2027430031 cites W2110350695 @default.
- W2027430031 cites W2110437274 @default.
- W2027430031 cites W2112072091 @default.
- W2027430031 cites W2115985987 @default.
- W2027430031 cites W2119943510 @default.
- W2027430031 cites W2123936150 @default.
- W2027430031 cites W2153285573 @default.
- W2027430031 cites W2156072461 @default.
- W2027430031 cites W2156968973 @default.
- W2027430031 cites W2158526532 @default.
- W2027430031 cites W2161009943 @default.
- W2027430031 cites W2233524970 @default.
- W2027430031 cites W2402860078 @default.
- W2027430031 cites W4243421010 @default.
- W2027430031 cites W4247935329 @default.
- W2027430031 doi "https://doi.org/10.1016/j.ab.2011.10.051" @default.
- W2027430031 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22119071" @default.
- W2027430031 hasPublicationYear "2012" @default.
- W2027430031 type Work @default.
- W2027430031 sameAs 2027430031 @default.
- W2027430031 citedByCount "9" @default.
- W2027430031 countsByYear W20274300312013 @default.
- W2027430031 countsByYear W20274300312014 @default.
- W2027430031 countsByYear W20274300312015 @default.
- W2027430031 countsByYear W20274300312016 @default.
- W2027430031 countsByYear W20274300312021 @default.
- W2027430031 countsByYear W20274300312022 @default.
- W2027430031 crossrefType "journal-article" @default.
- W2027430031 hasAuthorship W2027430031A5027320260 @default.
- W2027430031 hasAuthorship W2027430031A5070286775 @default.
- W2027430031 hasAuthorship W2027430031A5078945168 @default.
- W2027430031 hasAuthorship W2027430031A5082004578 @default.
- W2027430031 hasConcept C10390740 @default.
- W2027430031 hasConcept C179998833 @default.
- W2027430031 hasConcept C185592680 @default.
- W2027430031 hasConcept C2778016309 @default.
- W2027430031 hasConcept C2778163477 @default.
- W2027430031 hasConcept C2778718757 @default.
- W2027430031 hasConcept C2779718769 @default.
- W2027430031 hasConcept C2781471481 @default.
- W2027430031 hasConcept C43617362 @default.
- W2027430031 hasConcept C55493867 @default.
- W2027430031 hasConceptScore W2027430031C10390740 @default.
- W2027430031 hasConceptScore W2027430031C179998833 @default.
- W2027430031 hasConceptScore W2027430031C185592680 @default.
- W2027430031 hasConceptScore W2027430031C2778016309 @default.
- W2027430031 hasConceptScore W2027430031C2778163477 @default.
- W2027430031 hasConceptScore W2027430031C2778718757 @default.
- W2027430031 hasConceptScore W2027430031C2779718769 @default.
- W2027430031 hasConceptScore W2027430031C2781471481 @default.
- W2027430031 hasConceptScore W2027430031C43617362 @default.
- W2027430031 hasConceptScore W2027430031C55493867 @default.
- W2027430031 hasFunder F4320320912 @default.
- W2027430031 hasIssue "1" @default.
- W2027430031 hasLocation W20274300311 @default.
- W2027430031 hasLocation W20274300312 @default.
- W2027430031 hasOpenAccess W2027430031 @default.
- W2027430031 hasPrimaryLocation W20274300311 @default.
- W2027430031 hasRelatedWork W1986890454 @default.
- W2027430031 hasRelatedWork W1998455871 @default.
- W2027430031 hasRelatedWork W2027430031 @default.
- W2027430031 hasRelatedWork W2039375014 @default.
- W2027430031 hasRelatedWork W2059817333 @default.
- W2027430031 hasRelatedWork W2081388994 @default.