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• W2027431059 endingPage "213" @default.
• W2027431059 startingPage "208" @default.
• W2027431059 abstract "The DNA polymerase gene of African swine fever virus (ASFV) was mapped by marker rescue experiments using a phosphonoacetlc acid-resistant mutant and hybridization with an oligonucleotide probe designed from the most conserved motif of family B DNA polymerases. Viral DNA fragments mapping in this region were cloned and sequenced. An open reading frame coding for a 1244 aminoacid long peptide with a molecular mass of 142.5 kDa was determined from the sequence. A unique feature of ASFV DNA polymerase is the presence of 13 tandem repeats of the sequence Ala-Gly-Asp-Pro near the carboxyl end of the molecule. Comparison with 30 sequences of alpha-like DNA polymerases of cellular and viral origin showed that ASFV DNA polymerase has all the conserved motifs of family B DNA polymerases. A 3.9 kb transcript was detected by Northern hybridization and the transcription Initiation and termination sites were mapped by S1 analysis and primer extension. Late transcription was initiated at a site different from the early transcription initiation site. A 145 kDa protein, consistent with the size of the gene, was identified by an In situ enzyme assay after gel electrophoresis of Infected cell extracts." @default.
• W2027431059 created "2016-06-24" @default.
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• W2027431059 date "1994-01-01" @default.
• W2027431059 modified "2023-09-23" @default.
• W2027431059 title "Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus" @default.
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• W2027431059 doi "https://doi.org/10.1093/nar/22.2.208" @default.
• W2027431059 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/307773" @default.
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• W2027431059 hasPublicationYear "1994" @default.
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