FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027481001> ?p ?o ?g. }

• W2027481001 endingPage "16635" @default.
• W2027481001 startingPage "16623" @default.
• W2027481001 abstract "One of the earliest events in neural crest development takes place at the neural plate border and consists in the induction of Pax3 expression by posteriorizing Wnt·β-catenin signaling. The molecular mechanism of this regulation is not well understood, but several observations suggest a role for posteriorizing Cdx transcription factors (Cdx1/2/4) in this process. Cdx genes are known as integrators of posteriorizing signals from Wnt, retinoic acid, and FGF pathways. In this work, we report that Wnt-mediated regulation of murine Pax3 expression is indirect and involves Cdx proteins as intermediates. We show that Pax3 transcripts co-localize with Cdx proteins in the posterior neurectoderm and that neural Pax3 expression is reduced in Cdx1-null embryos. Using Wnt3a-treated P19 cells and neural crest-derived Neuro2a cells, we demonstrate that Pax3 expression is induced by the Wnt-Cdx pathway. Co-transfection analyses, electrophoretic mobility shift assays, chromatin immunoprecipitation, and transgenic studies further indicate that Cdx proteins operate via direct binding to an evolutionarily conserved neural crest enhancer of the Pax3 proximal promoter. Taken together, these results suggest a novel neural function for Cdx proteins within the gene regulatory network controlling neural crest development. One of the earliest events in neural crest development takes place at the neural plate border and consists in the induction of Pax3 expression by posteriorizing Wnt·β-catenin signaling. The molecular mechanism of this regulation is not well understood, but several observations suggest a role for posteriorizing Cdx transcription factors (Cdx1/2/4) in this process. Cdx genes are known as integrators of posteriorizing signals from Wnt, retinoic acid, and FGF pathways. In this work, we report that Wnt-mediated regulation of murine Pax3 expression is indirect and involves Cdx proteins as intermediates. We show that Pax3 transcripts co-localize with Cdx proteins in the posterior neurectoderm and that neural Pax3 expression is reduced in Cdx1-null embryos. Using Wnt3a-treated P19 cells and neural crest-derived Neuro2a cells, we demonstrate that Pax3 expression is induced by the Wnt-Cdx pathway. Co-transfection analyses, electrophoretic mobility shift assays, chromatin immunoprecipitation, and transgenic studies further indicate that Cdx proteins operate via direct binding to an evolutionarily conserved neural crest enhancer of the Pax3 proximal promoter. Taken together, these results suggest a novel neural function for Cdx proteins within the gene regulatory network controlling neural crest development." @default.
• W2027481001 created "2016-06-24" @default.
• W2027481001 creator A5014336180 @default.
• W2027481001 creator A5033200649 @default.
• W2027481001 creator A5059028677 @default.
• W2027481001 creator A5062259454 @default.
• W2027481001 creator A5066511911 @default.
• W2027481001 creator A5087482614 @default.
• W2027481001 date "2012-05-01" @default.
• W2027481001 modified "2023-10-16" @default.
• W2027481001 title "Caudal-related Homeobox (Cdx) Protein-dependent Integration of Canonical Wnt Signaling on Paired-box 3 (Pax3) Neural Crest Enhancer" @default.
• W2027481001 cites W110925524 @default.
• W2027481001 cites W1555019066 @default.
• W2027481001 cites W1819918826 @default.
• W2027481001 cites W1867052108 @default.
• W2027481001 cites W1892875901 @default.
• W2027481001 cites W1927233045 @default.
• W2027481001 cites W1937613866 @default.
• W2027481001 cites W1964920787 @default.
• W2027481001 cites W1965947444 @default.
• W2027481001 cites W1966566830 @default.
• W2027481001 cites W1967191615 @default.
• W2027481001 cites W1974648749 @default.
• W2027481001 cites W1977385412 @default.
• W2027481001 cites W1977926834 @default.
• W2027481001 cites W1978826904 @default.
• W2027481001 cites W1983245278 @default.
• W2027481001 cites W1985368135 @default.
• W2027481001 cites W1987865998 @default.
• W2027481001 cites W1988769766 @default.
• W2027481001 cites W1989770606 @default.
• W2027481001 cites W1993845489 @default.
• W2027481001 cites W1994144105 @default.
• W2027481001 cites W1994299057 @default.
• W2027481001 cites W1995485466 @default.
• W2027481001 cites W2001894458 @default.
• W2027481001 cites W2003880876 @default.
• W2027481001 cites W2006163287 @default.
• W2027481001 cites W2006591452 @default.
• W2027481001 cites W2008051450 @default.
• W2027481001 cites W2023353715 @default.
• W2027481001 cites W2025903960 @default.
• W2027481001 cites W2026046334 @default.
• W2027481001 cites W2029787649 @default.
• W2027481001 cites W2030940111 @default.
• W2027481001 cites W2032894029 @default.
• W2027481001 cites W2035510388 @default.
• W2027481001 cites W2038206901 @default.
• W2027481001 cites W2039113189 @default.
• W2027481001 cites W2039475510 @default.
• W2027481001 cites W2040398187 @default.
• W2027481001 cites W2041534255 @default.
• W2027481001 cites W2044715270 @default.
• W2027481001 cites W2046299900 @default.
• W2027481001 cites W2047271695 @default.
• W2027481001 cites W2052671006 @default.
• W2027481001 cites W2056208399 @default.
• W2027481001 cites W2064073950 @default.
• W2027481001 cites W2065546267 @default.
• W2027481001 cites W2067109855 @default.
• W2027481001 cites W2068701286 @default.
• W2027481001 cites W2072716955 @default.
• W2027481001 cites W2072838027 @default.
• W2027481001 cites W2077154296 @default.
• W2027481001 cites W2077720819 @default.
• W2027481001 cites W2078393889 @default.
• W2027481001 cites W2079978621 @default.
• W2027481001 cites W2080192511 @default.
• W2027481001 cites W2080342872 @default.
• W2027481001 cites W2082135441 @default.
• W2027481001 cites W2083838076 @default.
• W2027481001 cites W2088148296 @default.
• W2027481001 cites W2092909332 @default.
• W2027481001 cites W2095277280 @default.
• W2027481001 cites W2098342899 @default.
• W2027481001 cites W2098579354 @default.
• W2027481001 cites W2099073211 @default.
• W2027481001 cites W2102434502 @default.
• W2027481001 cites W2103841961 @default.
• W2027481001 cites W2106384420 @default.
• W2027481001 cites W2108201047 @default.
• W2027481001 cites W2108489942 @default.
• W2027481001 cites W2109577301 @default.
• W2027481001 cites W2112105989 @default.
• W2027481001 cites W2113284270 @default.
• W2027481001 cites W2114124747 @default.
• W2027481001 cites W2117265023 @default.
• W2027481001 cites W2117916454 @default.
• W2027481001 cites W2120662540 @default.
• W2027481001 cites W2121982298 @default.
• W2027481001 cites W2122506917 @default.
• W2027481001 cites W2124654191 @default.
• W2027481001 cites W2125856353 @default.
• W2027481001 cites W2128665076 @default.
• W2027481001 cites W2132842784 @default.
• W2027481001 cites W2134959578 @default.
• W2027481001 cites W2144172863 @default.
• W2027481001 cites W2144277963 @default.

• next