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- W2027599866 abstract "To develop new treatment possibilities for patients with severe lung diseases it is crucial to understand the lung functionon an alveolar level. Optical coherence tomography (OCT) in combination with intravital microscopy (IVM) are used forimaging subpleural alveoli in animal models to gain information about dynamic and morphological changes of lungtissue during mechanical ventilation. The image content suitable for further analysis is influenced by image artifactscaused by scattering, refraction, reflection, and absorbance. Because the refractive index varies with each air-tissueinterface in lung tissue, these effects decrease OCT image quality exceedingly. The quality of OCT images can beincreased when the refractive index inside the alveoli is matched to the one of tissue via liquid-filling. Thereby,scattering loss can be decreased and higher penetration depth and tissue contrast can be achieved. To use the advantagesof liquid-filling for in vivo imaging of small rodent lungs, a suitable breathing fluid (perfluorodecalin) and a specialliquid respirator are necessary. Here we show the effect of liquid-filling on OCT and IVM image quality of subpleuralalveoli in a mouse model." @default.
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- W2027599866 date "2012-02-09" @default.
- W2027599866 modified "2023-09-24" @default.
- W2027599866 title "Quantitative investigation of alveolar structures with OCT using total liquid ventilation during mechanical ventilation" @default.
- W2027599866 doi "https://doi.org/10.1117/12.908046" @default.
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