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- W2027608054 abstract "This paper describes a simple, rapid and economical method to preserve healthy plant tissues or those infected with phytoplasma for molecular diagnosis and analysis. After thermal treatments, clover proliferation (CP) phytoplasma DNA in periwinkle leaf samples, which was exposed to the microwave heat up to 480sec, remained intact but not after 600sec. The extracted DNA of the CP-infected tissues was amplified with phytoplasma universal primers showing a typical single band of 558bp in the microwave treated samples except the 600sec treatment, or healthy controls. Restriction digestions of PCR products of the heat treated samples with Alu I enzyme showed patterns similar to that with the digested PCR product of a fresh CP-infected sample on 5% polyacrylamide gel-electrophoresis, indicating its stability after the microwave heat treatment. The identity of CP phytoplasma in each heat-treated sample was confirmed by its amplification with specific primers forming a prominent band of 196bp which matched the size of the primer pair that was specific to CP phytoplasma." @default.
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- W2027608054 date "1995-01-01" @default.
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- W2027608054 title "Molecular Stability of Clover Proliferation Phytoplasma DNA in Periwinkle Plant Tissues after Thermal Treatment under Microwave Conditions." @default.
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- W2027608054 doi "https://doi.org/10.2183/pjab.71.265" @default.
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