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• W2027641887 abstract "OBJECTIVE: The first week of human development is marked by the differentiation of the early embryo into the inner cell mass (ICM) and trophectoderm (TE). Whereas the oocytes, embryos and human embryonic stem cells (hESC) derived from ICM, have been particularly studied, the TE is still largely unexplored. Here, we report the human TE transcriptome by whole genome expression profiling, and we compare this transciptome with that of the embryo on day-3.DESIGN: Five human TE samples (day-5) were separated from the ICM under dissecting microscope and donated embryos on day-3 were collected after informed consent from patients participating in our IVF program.MATERIALS AND METHODS: After total RNA extraction, amplification and hybridization, each sample was individually analyzed with Affymetrix Genechip human microarrays (HG-U133 Plus 2.0). Statistical analysis was carried out with Significance Analysis of Microarrays (SAM) method with 2-fold cut-off and false discovery rate (FDR < 5%).RESULTS: Out of 25,970 analysed transcripts a list of 910 genes were significantly over-expressed in TE samples. This TE molecular signature comprised genes known to be involved in murine TE biology such as GATA3 (x704, p<0.02), cytoskeleton protein keratin 18 (x524.2, p<0.02) and new candidates that can serve as markers for human TE health and many that contribute to the TE development. The very high expression level of extracellular matrix related LAMA1 (x324.3, p<0.02) in TE suggested that this laminin alpha1 could play an important role in TE specification. Conversely, human embryo on day-3 over-expressed numerous different zinc finger transcription factors, including ZNF595..CONCLUSION: The transcriptomic analysis of TE and embryos on day-3 improve our understanding of the molecular network controlling human trophoblast specification and pluripotency. In addition, the results establish a solid basis for the identification of potential biomarkers for blastocyst viability. OBJECTIVE: The first week of human development is marked by the differentiation of the early embryo into the inner cell mass (ICM) and trophectoderm (TE). Whereas the oocytes, embryos and human embryonic stem cells (hESC) derived from ICM, have been particularly studied, the TE is still largely unexplored. Here, we report the human TE transcriptome by whole genome expression profiling, and we compare this transciptome with that of the embryo on day-3. DESIGN: Five human TE samples (day-5) were separated from the ICM under dissecting microscope and donated embryos on day-3 were collected after informed consent from patients participating in our IVF program. MATERIALS AND METHODS: After total RNA extraction, amplification and hybridization, each sample was individually analyzed with Affymetrix Genechip human microarrays (HG-U133 Plus 2.0). Statistical analysis was carried out with Significance Analysis of Microarrays (SAM) method with 2-fold cut-off and false discovery rate (FDR < 5%). RESULTS: Out of 25,970 analysed transcripts a list of 910 genes were significantly over-expressed in TE samples. This TE molecular signature comprised genes known to be involved in murine TE biology such as GATA3 (x704, p<0.02), cytoskeleton protein keratin 18 (x524.2, p<0.02) and new candidates that can serve as markers for human TE health and many that contribute to the TE development. The very high expression level of extracellular matrix related LAMA1 (x324.3, p<0.02) in TE suggested that this laminin alpha1 could play an important role in TE specification. Conversely, human embryo on day-3 over-expressed numerous different zinc finger transcription factors, including ZNF595.. CONCLUSION: The transcriptomic analysis of TE and embryos on day-3 improve our understanding of the molecular network controlling human trophoblast specification and pluripotency. In addition, the results establish a solid basis for the identification of potential biomarkers for blastocyst viability." @default.
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• W2027641887 date "2010-09-01" @default.
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• W2027641887 title "Differential gene expression profiles between human embryo on day-3 and trophoblast cells on day-5: specific molecular signature" @default.
• W2027641887 doi "https://doi.org/10.1016/j.fertnstert.2010.07.082" @default.
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