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• W2027691522 startingPage "e3279" @default.
• W2027691522 abstract "Background Prefabricated expression microarrays are currently available for only a few species but methods have been proposed to extend their application to comparisons between divergent genomes. Methodology/Principal Findings Here we demonstrate that the hybridization intensity of genomic DNA is a poor basis on which to select unbiased probes on Affymetrix expression arrays for studies of comparative transcriptomics, and that doing so produces spurious results. We used the Affymetrix Xenopus laevis microarray to evaluate expression divergence between X. laevis, X. borealis, and their F1 hybrids. When data are analyzed with probes that interrogate only sequences with confirmed identity in both species, we recover results that differ substantially analyses that use genomic DNA hybridizations to select probes. Conclusions/Significance Our findings have implications for the experimental design of comparative expression studies that use single-species microarrays, and for our understanding of divergent expression in hybrid clawed frogs. These findings also highlight important limitations of single-species microarrays for studies of comparative transcriptomics of polyploid species." @default.
• W2027691522 created "2016-06-24" @default.
• W2027691522 creator A5046517948 @default.
• W2027691522 creator A5072205079 @default.
• W2027691522 creator A5081591244 @default.
• W2027691522 date "2008-09-25" @default.
• W2027691522 modified "2023-09-23" @default.
• W2027691522 title "Single-Species Microarrays and Comparative Transcriptomics" @default.
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• W2027691522 doi "https://doi.org/10.1371/journal.pone.0003279" @default.
• W2027691522 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2533705" @default.
• W2027691522 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18815615" @default.
• W2027691522 hasPublicationYear "2008" @default.
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