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- W2027720752 abstract "The gene for glial fibrillary acidic protein (GFAP) was analyzed in the rat for developmental changes in methylation of cytosine at CpG sequences as a correlate of the onset of GFAP mRNA expression and for the effect of methylation on GFAP promoter activity. The methylation of nine CpG sites in the GFAP promoter and ten sites in exon 1 was analyzed in F344 rats by a quantitative application of ligation-mediated polymerase chain reaction. Whole rat brain poly(A)+ RNA showed an exponential increase of GFAP mRNA after embryo day 14 that reached stable adult levels by postnatal day 10. During development, only the seven CpG sites in the far-upstream promoter showed large changes in methylation; these sites constitute the brain-specific domain of methylation described in adult rats (Teter et al: J Neurosci Res 39:680, 1994). These seven CpG sites showed a cycle of demethylation during the onset of GFAP transcription in the embryo (between embryonic day 14 and postnatal day 10) followed by remethylation at later postnatal ages when GFAP mRNA remains prevalent. The minimum levels of methylation across these CpG sites displayed a gradient with the lowest minima at the 3' sites. This demethylation/remethylation cycle is a novel phenomenon in DNA methylation during perinatal development. The demethylation/remethylation cycle during development was also shown by the opposite-strand cytosines. Two cytosines in this region that are conserved in rat and mouse also undergo the same demethylation/remethylation cycle in the mouse GFAP gene during development, implying evolutionary conservation and functional significance. As a further test of functional significance, a Luciferase reporter gene assay was evaluated in primary cultured astrocytes; the activity of the GFAP promoter was reduced when it was methylated at one or all CpG sites. Therefore, the GFAP promoter may be activated in rodent development by transient demethylation of a conserved brain-specific methylation domain." @default.
- W2027720752 created "2016-06-24" @default.
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- W2027720752 date "1996-07-01" @default.
- W2027720752 modified "2023-10-16" @default.
- W2027720752 title "Methylation of the glial fibrillary acidic protein gene shows novel biphasic changes during brain development" @default.
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- W2027720752 cites W1582426967 @default.
- W2027720752 cites W1646206347 @default.
- W2027720752 cites W1659635034 @default.
- W2027720752 cites W178589356 @default.
- W2027720752 cites W1832209337 @default.
- W2027720752 cites W1865680829 @default.
- W2027720752 cites W1904875888 @default.
- W2027720752 cites W1965718695 @default.
- W2027720752 cites W1969373767 @default.
- W2027720752 cites W1972740636 @default.
- W2027720752 cites W1973434441 @default.
- W2027720752 cites W1979474933 @default.
- W2027720752 cites W1980273804 @default.
- W2027720752 cites W1992124034 @default.
- W2027720752 cites W1993063708 @default.
- W2027720752 cites W1996524350 @default.
- W2027720752 cites W1998912417 @default.
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- W2027720752 cites W2004818887 @default.
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- W2027720752 cites W2017549569 @default.
- W2027720752 cites W2023130972 @default.
- W2027720752 cites W2027953381 @default.
- W2027720752 cites W2029630391 @default.
- W2027720752 cites W2030411252 @default.
- W2027720752 cites W2032365946 @default.
- W2027720752 cites W2035185214 @default.
- W2027720752 cites W2035939797 @default.
- W2027720752 cites W2037687495 @default.
- W2027720752 cites W2039067014 @default.
- W2027720752 cites W2046241907 @default.
- W2027720752 cites W2046819538 @default.
- W2027720752 cites W2046895191 @default.
- W2027720752 cites W2049551720 @default.
- W2027720752 cites W2057147504 @default.
- W2027720752 cites W2058077390 @default.
- W2027720752 cites W2058391066 @default.
- W2027720752 cites W2058397444 @default.
- W2027720752 cites W2059028264 @default.
- W2027720752 cites W2059994807 @default.
- W2027720752 cites W2063006038 @default.
- W2027720752 cites W2064368446 @default.
- W2027720752 cites W2064919591 @default.
- W2027720752 cites W2072812579 @default.
- W2027720752 cites W2075942120 @default.
- W2027720752 cites W2077267135 @default.
- W2027720752 cites W2079366888 @default.
- W2027720752 cites W2082124034 @default.
- W2027720752 cites W2086524534 @default.
- W2027720752 cites W2091632562 @default.
- W2027720752 cites W2104457577 @default.
- W2027720752 cites W2111368349 @default.
- W2027720752 cites W2115534715 @default.
- W2027720752 cites W2121891818 @default.
- W2027720752 cites W2126323818 @default.
- W2027720752 cites W2129387586 @default.
- W2027720752 cites W2131484652 @default.
- W2027720752 cites W2140732424 @default.
- W2027720752 cites W2146517310 @default.
- W2027720752 cites W2162887181 @default.
- W2027720752 cites W24659260 @default.
- W2027720752 cites W4294216491 @default.
- W2027720752 cites W59685088 @default.
- W2027720752 doi "https://doi.org/10.1002/(sici)1098-1136(199607)17:3<195::aid-glia2>3.0.co;2-0" @default.
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