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• W2027752681 endingPage "207" @default.
• W2027752681 startingPage "199" @default.
• W2027752681 abstract "Abstract: The kinetics of GM-CSF and G-CSF secretion by purified adherent human monocytes were studied by quantitative immunoassays. Interleukin-1 (IL-1); 4–40 ng/ml and E. coli lipopolysaccharide (LPS); 0.1–100 ng/ml, were the most effective stimuli and induced dose-dependent secretion of both GM-CSF and G-CSF. Secretion of newly synthesized CSF was detectable 3–6 hours after stimulation and continued for approximately 24 h. Twenty minutes pulse exposure to LPS was sufficient to induce half maximum secretion of GM-CSF, and after 24–36 h the adherent monocytes could not be restimulated. Neither GM-CSF nor TNF could down-regulate the secretion of GM-CSF. IL-3 induced a minor secretion of GM-CSF whereas TNF, G-CSF, M-CSF and IFN-gamma were unable to induce GM-CSF secretion. In addition to LPS and IL-1, GM-CSF and to a minor degree TNF induced G-CSF secretion. Enriched T lymphocytes secreted GM-CSF, but not G-CSF, after stimulation with PHA or staphylococcal enterotoxin A (SEA), whereas LPS and IL-1 were without stimulatory effects. We also noted that enriched T lymphocytes added to LPS-stimulated adherent monocytes at ratios of 1:10 or more inhibited, in a dose-dependent fashion, GM-CSF secretion by 13–55%. These findings add new quantitative data on CSF secretion by human monocytes." @default.
• W2027752681 created "2016-06-24" @default.
• W2027752681 creator A5038829375 @default.
• W2027752681 creator A5075373587 @default.
• W2027752681 date "2009-04-24" @default.
• W2027752681 modified "2023-10-18" @default.
• W2027752681 title "Granulocyte-macrophage colony-stimulating factor (GM-CSF) and granulocyte colony-stimulating factor (G-CSF) secretion by adherent monocytes measured by quantitative immunoassays" @default.
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• W2027752681 doi "https://doi.org/10.1111/j.1600-0609.1992.tb00047.x" @default.
• W2027752681 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1281454" @default.
• W2027752681 hasPublicationYear "2009" @default.
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