FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2027772980> ?p ?o ?g. }

• W2027772980 abstract "Flt3 Ligand (FL) has been shown to elicit antitumor responses induced by tumor antigen stimulation. Allogeneic and xenogeneic cell lines transduced with cytokine genes may be used to augment the antitumor efficacy of tumor antigens.The objective was to evaluate the augmentation of tumor lysate-induced immunity by a more clinically applicable FL gene-transduced xenogeneic cell line in combination with interleukin-2 (IL-2) in a CC-36 murine colon cancer model.Human 143B osteosarcoma tumor cells were transduced with full-length FL cDNA (143B-FL). Secretion of FL from 143B-FL was analyzed in vivo in normal BALB/c mice transplanted with 143B-FL, and expansion of dendritic cells (DC) was also analyzed in the same mice by flow cytometry. Eight-week-old, male BALB/c mice were used in a prophylactic vaccination protocol utilizing tumor lysate (CLy), 143B-FL, and soluble IL-2. Prophylactic group designations (n = 10/group) were as follows: ten million 143B-FL cells (alone, with tumor lysate, or with tumor lysate and IL-2), IL-2 with tumor lysate, IL-2 alone, or a no treatment control. The tumor lysate (200 microg of protein) and IL-2 (100,000 IU) injections were administered intraperitoneally. Mice were challenged subcutaneously with 10(3) CC-36 tumor cells. Tumor protection and tumor burden (TB), as mean tumor diameter, were determined. Peripheral blood lymphocytes (PBLs) from the 143B-FL + IL-2 + tumor lysate vaccinated group were analyzed for cytolytic activity in 4-h chromium release assays. In addition, plasma cytokine concentrations of interleukin-12 (IL-12) and interferon gamma (IFN-gamma) were assessed by ELISA. Student's t tests were used for all statistical comparisons.In vivo expression of FL was observed 24 h following the inoculation of 143B-FL, and a four fold increase in DCs was observed in the peripheral blood of these mice. Mice immunized with a combination of 143B-FL, tumor lysate and IL-2 showed statistically significant protection against tumor development (10%) for 100 days after tumor challenge; incidences in other groups ranged from 40 to 100% (P < 0.05). Moreover, this immunization protocol produced the lowest TB at 3- and 6-week time points (0, 1.6 mm) when compared to all other groups (TB between 7.2 and 15.9 mm) (P < 0.05). In addition, PBLs from vaccinated mice showed increased cytolytic activity against CC-36 target cells. This corresponded to increased levels of IL-12 and IFN-g in the plasma of mice following vaccination.These data suggest that FL gene-transduced xenogeneic tumor cells may augment the immunity induced by tumor antigens and systemic IL-2 through the activation of dendritic cells and T-cell-mediated mechanisms." @default.
• W2027772980 created "2016-06-24" @default.
• W2027772980 creator A5003552161 @default.
• W2027772980 creator A5044336024 @default.
• W2027772980 creator A5076206620 @default.
• W2027772980 creator A5078321374 @default.
• W2027772980 date "2002-11-01" @default.
• W2027772980 modified "2023-09-27" @default.
• W2027772980 title "Adjuvant Effect of a Flt3 Ligand (FL) Gene-Transduced Xenogeneic Cell Line in a Murine Colon Cancer Model" @default.
• W2027772980 cites W1461314687 @default.
• W2027772980 cites W1489827712 @default.
• W2027772980 cites W1493941926 @default.
• W2027772980 cites W1505013360 @default.
• W2027772980 cites W1545819550 @default.
• W2027772980 cites W1560416396 @default.
• W2027772980 cites W1560562369 @default.
• W2027772980 cites W1604283084 @default.
• W2027772980 cites W1605948149 @default.
• W2027772980 cites W1787716617 @default.
• W2027772980 cites W1822927306 @default.
• W2027772980 cites W1840936814 @default.
• W2027772980 cites W1843453924 @default.
• W2027772980 cites W1845740517 @default.
• W2027772980 cites W1989722428 @default.
• W2027772980 cites W1990159457 @default.
• W2027772980 cites W1995809929 @default.
• W2027772980 cites W2012148175 @default.
• W2027772980 cites W2015266578 @default.
• W2027772980 cites W2017517342 @default.
• W2027772980 cites W2022262230 @default.
• W2027772980 cites W2025350367 @default.
• W2027772980 cites W2052877553 @default.
• W2027772980 cites W2053831142 @default.
• W2027772980 cites W2066925699 @default.
• W2027772980 cites W2071432029 @default.
• W2027772980 cites W2081332728 @default.
• W2027772980 cites W2084390253 @default.
• W2027772980 cites W2087664594 @default.
• W2027772980 cites W2094073817 @default.
• W2027772980 cites W2096869493 @default.
• W2027772980 cites W2106883237 @default.
• W2027772980 cites W2111220263 @default.
• W2027772980 cites W2117884326 @default.
• W2027772980 cites W2123070168 @default.
• W2027772980 cites W2128390662 @default.
• W2027772980 cites W2138463196 @default.
• W2027772980 cites W2142186946 @default.
• W2027772980 cites W2143671588 @default.
• W2027772980 cites W2156831625 @default.
• W2027772980 cites W2157154128 @default.
• W2027772980 cites W2160198088 @default.
• W2027772980 cites W2160257892 @default.
• W2027772980 cites W2164838433 @default.
• W2027772980 cites W2169539795 @default.
• W2027772980 cites W2170748847 @default.
• W2027772980 cites W2170933182 @default.
• W2027772980 cites W2177327503 @default.
• W2027772980 cites W2180137130 @default.
• W2027772980 cites W2208532882 @default.
• W2027772980 cites W2236607890 @default.
• W2027772980 cites W2312886182 @default.
• W2027772980 cites W2325666068 @default.
• W2027772980 cites W232742645 @default.
• W2027772980 cites W2330721411 @default.
• W2027772980 cites W2337362637 @default.
• W2027772980 cites W2339666810 @default.
• W2027772980 cites W2354001031 @default.
• W2027772980 cites W2408984161 @default.
• W2027772980 cites W79123086 @default.
• W2027772980 cites W1568922548 @default.
• W2027772980 doi "https://doi.org/10.1006/jsre.2002.6540" @default.
• W2027772980 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12443727" @default.
• W2027772980 hasPublicationYear "2002" @default.
• W2027772980 type Work @default.
• W2027772980 sameAs 2027772980 @default.
• W2027772980 citedByCount "8" @default.
• W2027772980 countsByYear W20277729802012 @default.
• W2027772980 crossrefType "journal-article" @default.
• W2027772980 hasAuthorship W2027772980A5003552161 @default.
• W2027772980 hasAuthorship W2027772980A5044336024 @default.
• W2027772980 hasAuthorship W2027772980A5076206620 @default.
• W2027772980 hasAuthorship W2027772980A5078321374 @default.
• W2027772980 hasConcept C147483822 @default.
• W2027772980 hasConcept C203014093 @default.
• W2027772980 hasConcept C2777701055 @default.
• W2027772980 hasConcept C2777863537 @default.
• W2027772980 hasConcept C2778690821 @default.
• W2027772980 hasConcept C502942594 @default.
• W2027772980 hasConcept C553184892 @default.
• W2027772980 hasConcept C71924100 @default.
• W2027772980 hasConcept C8891405 @default.
• W2027772980 hasConceptScore W2027772980C147483822 @default.
• W2027772980 hasConceptScore W2027772980C203014093 @default.
• W2027772980 hasConceptScore W2027772980C2777701055 @default.
• W2027772980 hasConceptScore W2027772980C2777863537 @default.
• W2027772980 hasConceptScore W2027772980C2778690821 @default.
• W2027772980 hasConceptScore W2027772980C502942594 @default.
• W2027772980 hasConceptScore W2027772980C553184892 @default.
• W2027772980 hasConceptScore W2027772980C71924100 @default.
• W2027772980 hasConceptScore W2027772980C8891405 @default.

• next