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• W2027830088 endingPage "777" @default.
• W2027830088 startingPage "761" @default.
• W2027830088 abstract "Summary The anaerobic bacterium Clostridium perfringens mediates clostridial myonecrosis, or gas gangrene, by producing a number of extracellular toxins and enzymes. Transposon mutagenesis with Tn916 was used to isolate a pleiotropic mutant of C. perfringens that produced reduced levels of phospholipase C, protease and sialidase, and did not produce any detectable perfringolysin O activity. Southern hybridization revealed that a single copy of Tn 916 had inserted into a 2.7 kb Hin dlll fragment in the C. perfringens chromosome. A 4.3 kb Pstl fragment, which spanned the Tn 916 insertion site, was cloned from the wild‐type strain. When subcloned into a shuttle vector and introduced into C. perfringens this fragment was able to complement the Tn916‐derived mutation. Transformation of the mutant with plasmids containing the 2.7 kb Hin dlll fragment, or the 4.3 kb Pst l fragment resulted in toxin and enzyme levels greater than or equal to those of the wild‐type strain. The Pst l fragment was sequenced and found to potentially encode seven open reading frames, two of which appeared to be arranged in an operon and shared sequence similarity with members of two‐component signal transduction systems. The putative virR gene encoded a protein with a deduced molecular weight of 30140, and with sequence similarity to activators in the response regulator family of proteins. The next gene, virS , into which Tn 916 had inserted, was predicted to encode a membrane‐spanning protein with a deduced molecular weight of 51 274. The putative VirS protein had sequence similarity to sensor proteins and also contained a histidine residue highly conserved in the histidine protein kinase family of sensor proteins. Virulence studies carried out using a mouse model implicated the virS gene in the pathogenesis of histotoxic C. perfringens infections. It was concluded that a two‐component sensor regulator system that activated the expression of a number of extracellular toxins and enzymes involved In virulence had been cloned and sequenced. A model that described the regulation of extracellular toxin production in C. perfringens was constructed." @default.
• W2027830088 created "2016-06-24" @default.
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• W2027830088 date "1994-06-01" @default.
• W2027830088 modified "2023-10-09" @default.
• W2027830088 title "Identification and molecular analysis of a locus that regulates extracellular toxin production in <i>Clostridium perfringens</i>" @default.
• W2027830088 cites W127841036 @default.
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• W2027830088 cites W1486622462 @default.
• W2027830088 cites W1517473122 @default.
• W2027830088 cites W1522266198 @default.
• W2027830088 cites W1534005932 @default.
• W2027830088 cites W1540797945 @default.
• W2027830088 cites W1555480421 @default.
• W2027830088 cites W1586777183 @default.
• W2027830088 cites W1653505145 @default.
• W2027830088 cites W1670230410 @default.
• W2027830088 cites W1713375687 @default.
• W2027830088 cites W1775749144 @default.
• W2027830088 cites W1790927204 @default.
• W2027830088 cites W1820944463 @default.
• W2027830088 cites W1826396019 @default.
• W2027830088 cites W1833346845 @default.
• W2027830088 cites W1833883636 @default.
• W2027830088 cites W1842286347 @default.
• W2027830088 cites W1849438622 @default.
• W2027830088 cites W1865912013 @default.
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• W2027830088 cites W1993726268 @default.
• W2027830088 cites W1995962099 @default.
• W2027830088 cites W2016585890 @default.
• W2027830088 cites W2016844373 @default.
• W2027830088 cites W2021547065 @default.
• W2027830088 cites W2025763720 @default.
• W2027830088 cites W2026462493 @default.
• W2027830088 cites W2032470595 @default.
• W2027830088 cites W2033340647 @default.
• W2027830088 cites W2036196278 @default.
• W2027830088 cites W2044688143 @default.
• W2027830088 cites W2046913779 @default.
• W2027830088 cites W2048205348 @default.
• W2027830088 cites W2049583838 @default.
• W2027830088 cites W2051689433 @default.
• W2027830088 cites W2051830305 @default.
• W2027830088 cites W2051906313 @default.
• W2027830088 cites W2054941036 @default.
• W2027830088 cites W2055043387 @default.
• W2027830088 cites W2058979757 @default.
• W2027830088 cites W2059438383 @default.
• W2027830088 cites W2063643907 @default.
• W2027830088 cites W2090070554 @default.
• W2027830088 cites W2090672353 @default.
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• W2027830088 cites W2108141139 @default.
• W2027830088 cites W2120404655 @default.
• W2027830088 cites W2120684043 @default.
• W2027830088 cites W2127096639 @default.
• W2027830088 cites W2133521178 @default.
• W2027830088 cites W2140620497 @default.
• W2027830088 cites W2143052983 @default.
• W2027830088 cites W2143115044 @default.
• W2027830088 cites W2143234559 @default.
• W2027830088 cites W2144112248 @default.
• W2027830088 cites W2155825582 @default.
• W2027830088 cites W2169679742 @default.
• W2027830088 cites W2170509240 @default.
• W2027830088 cites W4245067683 @default.
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• W2027830088 doi "https://doi.org/10.1111/j.1365-2958.1994.tb01063.x" @default.
• W2027830088 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8052128" @default.
• W2027830088 hasPublicationYear "1994" @default.
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