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• W2027831318 endingPage "9953" @default.
• W2027831318 startingPage "9947" @default.
• W2027831318 abstract "Cationic polysaccharides based on spermine−dextran conjugates were synthesized and tested as vectors for gene transfection. Dextrans of 10−380 kDa were oxidized under mild conditions by potassium periodate to obtain the respective polyaldehydes in 90% overall yield. The oxidized dextrans were reacted by reductive amination with increasing amounts of spermine, and the efficacy of conjugation between the oligoamine and polysaccharides was studied as a function of spermine/aldehyde mole ratio, pH, and temperature of medium. The optimal conjugation yields were obtained at 1.25 mole ratio (spermine/aldehyde groups) and pH 11 at room temperature. Under these conditions, ∼2 μmol/mg (spermine/polysaccharide) conjugation was achieved with 25−30% of the spermine moieties were conjugated in both sides to form branched polymers. The water-soluble polymers obtained were interacted with pCMV-GFP plasmid to form nanoparticles that were introduced to HEK293 and NIH3T3 cells in vitro for transfection efficacy assessment. Out of about 50 different polymer structures, only spermine−dextran of 6000−8000 Da, spermine content of ∼2 μmol/mg, and degree of branching of 25−30% was active in transfecting about 50% of the cells while all other polymers were significantly less active." @default.
• W2027831318 created "2016-06-24" @default.
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• W2027831318 date "2002-12-01" @default.
• W2027831318 modified "2023-09-27" @default.
• W2027831318 title "Cationic Polysaccharides for Gene Delivery" @default.
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• W2027831318 doi "https://doi.org/10.1021/ma0209592" @default.
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