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- W2027831345 abstract "A total of sixteen recombinant variants of a major house dust mite allergen Der f 2, the wild-type Der f 2, six cysteine mutants, six proline mutants, and three lysine mutants, were expressed in Escherichia coli. The cells were solubilized and run on SDS-PAGE under reducing conditions. Epitopes for five mouse anti-Der f 2 monoclonal antibodies, 1B2, 7C10, 13A4, 15E11, and 18G8, to the recombinant Der f 2 variants were characterized by immunoblot analysis." @default.
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- W2027831345 date "2001-01-01" @default.
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- W2027831345 title "Reactivities of Mutants of a Major House Dust Mite Allergen Der f 2 to Mouse Anti-Der f 2 Monoclonal Antibodies Analyzed by Immunoblotting" @default.
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- W2027831345 doi "https://doi.org/10.1271/bbb.65.694" @default.
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