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• W2027853792 abstract "Experiments were carried out to identify a process co-determining with QA the fluorescence rise between F0 and FM. With 3-(3′,4′-dichlorophenyl)-1,1-dimethylurea (DCMU), the fluorescence rise is sigmoidal, in its absence it is not. Lowering the temperature to − 10 °C the sigmoidicity is lost. It is shown that the sigmoidicity is due to the kinetic overlap between the reduction kinetics of QA and a second process; an overlap that disappears at low temperature because the temperature dependences of the two processes differ. This second process can still relax at − 60 °C where recombination between QA− and the donor side of photosystem (PS) II is blocked. This suggests that it is not a redox reaction but a conformational change can explain the data. Without DCMU, a reduced photosynthetic electron transport chain (ETC) is a pre-condition for reaching the FM. About 40% of the variable fluorescence relaxes in 100 ms. Re-induction while the ETC is still reduced takes a few ms and this is a photochemical process. The fact that the process can relax and be re-induced in the absence of changes in the redox state of the plastoquinone (PQ) pool implies that it is unrelated to the QB-occupancy state and PQ-pool quenching. In both +/−DCMU the process studied represents ~ 30% of the fluorescence rise. The presented observations are best described within a conformational protein relaxation concept. In untreated leaves we assume that conformational changes are only induced when QA is reduced and relax rapidly on re-oxidation. This would explain the relationship between the fluorescence rise and the ETC-reduction." @default.
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• W2027853792 date "2009-09-01" @default.
• W2027853792 modified "2023-09-23" @default.
• W2027853792 title "9316 Follow-up study in high-risk uveal melanoma patients" @default.
• W2027853792 doi "https://doi.org/10.1016/s1359-6349(09)71960-3" @default.
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