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- W2027910079 abstract "The great usefulness of the previously reported stopped-flow time difference analysis technique for improving enzymatic analyses based on dehydrogenase-catalyzed reactions is demonstrated by combining the technique with the conventional enzymatic method for pyruvate determination. Reagent economy was accomplished by using a micro-stopped-flow apparatus designed by one of the authors. The running speed and sensitivity were improved by about 100 and 7 times the figures for the conventional enzymatic method, respectively. Furthermore, as procedural blanks were unnecessary, a simpler method could be developed. The interference by several other keto acids was easily eliminated by analyzing the reaction curve, and the apparent first-order rate constant proved to be useful for qualitative analysis." @default.
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- W2027910079 date "1988-01-01" @default.
- W2027910079 modified "2023-09-27" @default.
- W2027910079 title "Application of the micro-stopped-flow time difference analysis technique to a dehydrogenase-catalyzed reaction for rapid and sensitive determination of pyruvate." @default.
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- W2027910079 doi "https://doi.org/10.1271/bbb1961.52.2563" @default.
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