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- W2028002005 abstract "The expression of protein tyrosine phosphatases (PTPs) was examined in preimplantation mouse embryos. PTPs expressed in the various developmental stages, including unfertilized oocytes, 2-cell, 4-cell, morula and blastocyst stages, were identified by reverse transcription of mRNA followed by a polymerase chain reaction (RT-PCR) with degenerate oligonucleotide primers corresponding to the conserved catalytic domain of PTP. Sequencing of cDNA clones obtained by RT-PCR revealed the expression of mRPTPB, SHP-1, PTPT9, SHP-2 and LAR. The changes in the expression levels of these PTPs during preimplantation development were examined by RT-PCR using each gene specific primers. The results showed that their expression levels dynamically changed in different manners among PTPs, suggesting that PTPs play roles in the regulation of growth and differentiation during preimplantation development." @default.
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- W2028002005 date "2001-01-01" @default.
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- W2028002005 title "Expression of Protein Tyrosine Phosphatases in the Preimplantation Mouse Embryos" @default.
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- W2028002005 doi "https://doi.org/10.2508/chikusan.72.404" @default.
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