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- W2028016637 endingPage "e28485" @default.
- W2028016637 startingPage "e28485" @default.
- W2028016637 abstract "Brucella melitensis is an important zoonotic pathogen that causes brucellosis, a disease that affects sheep, cattle and occasionally humans. B. melitensis strain M5-90, a live attenuated vaccine cultured from B. melitensis strain M28, has been used as an effective tool in the control of brucellosis in goats and sheep in China. However, the molecular changes leading to attenuated virulence and pathogenicity in B. melitensis remain poorly understood. In this study we employed the Illumina Genome Analyzer platform to perform genome-wide digital gene expression (DGE) analysis of mouse peritoneal macrophage responses to B. melitensis infection. Many parallel changes in gene expression profiles were observed in M28- and M5-90-infected macrophages, suggesting that they employ similar survival strategies, notably the induction of anti-inflammatory and antiapoptotic factors. Moreover, 1019 differentially expressed macrophage transcripts were identified 4 h after infection with the different B. melitensis strains, and these differential transcripts notably identified genes involved in the lysosome and mitogen-activated protein kinase (MAPK) pathways. Further analysis employed gene ontology (GO) analysis: high-enrichment GOs identified endocytosis, inflammatory, apoptosis, and transport pathways. Path-Net and Signal-Net analysis highlighted the MAPK pathway as the key regulatory pathway. Moreover, the key differentially expressed genes of the significant pathways were apoptosis-related. These findings demonstrate previously unrecognized changes in gene transcription that are associated with B. melitensis infection of macrophages, and the central signaling pathways identified here merit further investigation. Our data provide new insights into the molecular attenuation mechanism of strain M5-90 and will facilitate the generation of new attenuated vaccine strains with enhanced efficacy." @default.
- W2028016637 created "2016-06-24" @default.
- W2028016637 creator A5023098151 @default.
- W2028016637 creator A5030660959 @default.
- W2028016637 creator A5055423740 @default.
- W2028016637 creator A5065503231 @default.
- W2028016637 creator A5071670007 @default.
- W2028016637 creator A5091807518 @default.
- W2028016637 date "2011-12-28" @default.
- W2028016637 modified "2023-09-24" @default.
- W2028016637 title "Deep-Sequencing Analysis of the Mouse Transcriptome Response to Infection with Brucella melitensis Strains of Differing Virulence" @default.
- W2028016637 cites W1528562972 @default.
- W2028016637 cites W1596515083 @default.
- W2028016637 cites W1796586970 @default.
- W2028016637 cites W1914905091 @default.
- W2028016637 cites W1964198812 @default.
- W2028016637 cites W1966486838 @default.
- W2028016637 cites W1967093917 @default.
- W2028016637 cites W1967111256 @default.
- W2028016637 cites W1968051713 @default.
- W2028016637 cites W1968793608 @default.
- W2028016637 cites W1969050107 @default.
- W2028016637 cites W1973748344 @default.
- W2028016637 cites W1980220254 @default.
- W2028016637 cites W1981509058 @default.
- W2028016637 cites W1991218047 @default.
- W2028016637 cites W1993763219 @default.
- W2028016637 cites W2003060606 @default.
- W2028016637 cites W2005452437 @default.
- W2028016637 cites W2006931894 @default.
- W2028016637 cites W2008869198 @default.
- W2028016637 cites W2013216100 @default.
- W2028016637 cites W2015649802 @default.
- W2028016637 cites W2022603493 @default.
- W2028016637 cites W2023275569 @default.
- W2028016637 cites W2034185443 @default.
- W2028016637 cites W2035028585 @default.
- W2028016637 cites W2043287922 @default.
- W2028016637 cites W2046585193 @default.
- W2028016637 cites W2047765648 @default.
- W2028016637 cites W2058501774 @default.
- W2028016637 cites W2061439657 @default.
- W2028016637 cites W2062123429 @default.
- W2028016637 cites W2063985472 @default.
- W2028016637 cites W2066791085 @default.
- W2028016637 cites W2067384813 @default.
- W2028016637 cites W2070166036 @default.
- W2028016637 cites W2070522972 @default.
- W2028016637 cites W2072403854 @default.
- W2028016637 cites W2077126724 @default.
- W2028016637 cites W2082069732 @default.
- W2028016637 cites W2082710846 @default.
- W2028016637 cites W2083309357 @default.
- W2028016637 cites W2083742560 @default.
- W2028016637 cites W2086438756 @default.
- W2028016637 cites W2095009390 @default.
- W2028016637 cites W2097754561 @default.
- W2028016637 cites W2098259424 @default.
- W2028016637 cites W2098478833 @default.
- W2028016637 cites W2100625056 @default.
- W2028016637 cites W2103989683 @default.
- W2028016637 cites W2104073328 @default.
- W2028016637 cites W2104918497 @default.
- W2028016637 cites W2105389530 @default.
- W2028016637 cites W2106432984 @default.
- W2028016637 cites W2114908063 @default.
- W2028016637 cites W2116166762 @default.
- W2028016637 cites W2119752906 @default.
- W2028016637 cites W2121008420 @default.
- W2028016637 cites W2121182539 @default.
- W2028016637 cites W2121325716 @default.
- W2028016637 cites W2127635667 @default.
- W2028016637 cites W2131162745 @default.
- W2028016637 cites W2134388096 @default.
- W2028016637 cites W2135233980 @default.
- W2028016637 cites W2141009231 @default.
- W2028016637 cites W2144099695 @default.
- W2028016637 cites W2145646914 @default.
- W2028016637 cites W2146516445 @default.
- W2028016637 cites W2147030408 @default.
- W2028016637 cites W2149200346 @default.
- W2028016637 cites W2150070236 @default.
- W2028016637 cites W2150500173 @default.
- W2028016637 cites W2150926065 @default.
- W2028016637 cites W2152423594 @default.
- W2028016637 cites W2152495216 @default.
- W2028016637 cites W2158966093 @default.
- W2028016637 cites W2159686118 @default.
- W2028016637 cites W2159944158 @default.
- W2028016637 cites W2170392236 @default.
- W2028016637 cites W2170414736 @default.
- W2028016637 cites W2170528209 @default.
- W2028016637 cites W2402457099 @default.
- W2028016637 doi "https://doi.org/10.1371/journal.pone.0028485" @default.
- W2028016637 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3247208" @default.
- W2028016637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22216095" @default.
- W2028016637 hasPublicationYear "2011" @default.
- W2028016637 type Work @default.