Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028041504> ?p ?o ?g. }
- W2028041504 endingPage "12499" @default.
- W2028041504 startingPage "12489" @default.
- W2028041504 abstract "The transmembrane serine protease MarP is important for pH homeostasis in Mycobacterium tuberculosis (Mtb). Previous structural studies revealed that MarP contains a chymotrypsin fold and a disulfide bond that stabilizes the protease active site in the substrate-bound conformation. Here, we determined that MarP is located in the Mtb periplasm and showed that this localization is essential for function. Using the recombinant protease domain of MarP, we identified its substrate specificity using two independent assays: positional-scanning synthetic combinatorial library profiling and multiplex substrate profiling by mass spectrometry. These methods revealed that MarP prefers bulky residues at P4, tryptophan or leucine at P2, arginine or hydrophobic residues at P1, and alanine or asparagine at P1'. Guided by these data, we designed fluorogenic peptide substrates and characterized the kinetic properties of MarP. Finally, we tested the impact of mutating MarP cysteine residues on the peptidolytic activity of recombinant MarP and its ability to complement phenotypes of Mtb ΔMarP. Taken together, our studies provide insight into the enzymatic properties of MarP, its substrate preference, and the importance of its transmembrane helices and disulfide bond." @default.
- W2028041504 created "2016-06-24" @default.
- W2028041504 creator A5016119935 @default.
- W2028041504 creator A5023886894 @default.
- W2028041504 creator A5038194767 @default.
- W2028041504 creator A5039579571 @default.
- W2028041504 creator A5046154621 @default.
- W2028041504 creator A5069716975 @default.
- W2028041504 creator A5085424463 @default.
- W2028041504 creator A5087001996 @default.
- W2028041504 date "2013-05-01" @default.
- W2028041504 modified "2023-10-12" @default.
- W2028041504 title "Substrate Specificity of MarP, a Periplasmic Protease Required for Resistance to Acid and Oxidative Stress in Mycobacterium tuberculosis" @default.
- W2028041504 cites W1740666950 @default.
- W2028041504 cites W1831560899 @default.
- W2028041504 cites W1966448483 @default.
- W2028041504 cites W1972269365 @default.
- W2028041504 cites W1987273310 @default.
- W2028041504 cites W1989883893 @default.
- W2028041504 cites W1992583354 @default.
- W2028041504 cites W1994149926 @default.
- W2028041504 cites W2001197606 @default.
- W2028041504 cites W2010961842 @default.
- W2028041504 cites W2012506575 @default.
- W2028041504 cites W2012845953 @default.
- W2028041504 cites W2022725992 @default.
- W2028041504 cites W2026405416 @default.
- W2028041504 cites W2028685242 @default.
- W2028041504 cites W2040474140 @default.
- W2028041504 cites W2041823495 @default.
- W2028041504 cites W2042126350 @default.
- W2028041504 cites W2044582322 @default.
- W2028041504 cites W2047930189 @default.
- W2028041504 cites W2050642108 @default.
- W2028041504 cites W2053855749 @default.
- W2028041504 cites W2054705564 @default.
- W2028041504 cites W2055600356 @default.
- W2028041504 cites W2058677329 @default.
- W2028041504 cites W2064080654 @default.
- W2028041504 cites W2065542724 @default.
- W2028041504 cites W2081589091 @default.
- W2028041504 cites W2090197974 @default.
- W2028041504 cites W2091273971 @default.
- W2028041504 cites W2098942474 @default.
- W2028041504 cites W2100104025 @default.
- W2028041504 cites W2103700658 @default.
- W2028041504 cites W2108073693 @default.
- W2028041504 cites W2116053973 @default.
- W2028041504 cites W2119784689 @default.
- W2028041504 cites W2120387439 @default.
- W2028041504 cites W2122976061 @default.
- W2028041504 cites W2136000113 @default.
- W2028041504 cites W2139603922 @default.
- W2028041504 cites W2144081223 @default.
- W2028041504 cites W2145684516 @default.
- W2028041504 cites W2147251439 @default.
- W2028041504 cites W2153290883 @default.
- W2028041504 cites W2154926401 @default.
- W2028041504 cites W2155767187 @default.
- W2028041504 cites W2158023121 @default.
- W2028041504 cites W2163990363 @default.
- W2028041504 cites W2165824222 @default.
- W2028041504 cites W2166305071 @default.
- W2028041504 cites W2171702377 @default.
- W2028041504 cites W336065844 @default.
- W2028041504 cites W4241265708 @default.
- W2028041504 doi "https://doi.org/10.1074/jbc.m113.456541" @default.
- W2028041504 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3642297" @default.
- W2028041504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23504313" @default.
- W2028041504 hasPublicationYear "2013" @default.
- W2028041504 type Work @default.
- W2028041504 sameAs 2028041504 @default.
- W2028041504 citedByCount "31" @default.
- W2028041504 countsByYear W20280415042013 @default.
- W2028041504 countsByYear W20280415042014 @default.
- W2028041504 countsByYear W20280415042015 @default.
- W2028041504 countsByYear W20280415042016 @default.
- W2028041504 countsByYear W20280415042017 @default.
- W2028041504 countsByYear W20280415042018 @default.
- W2028041504 countsByYear W20280415042019 @default.
- W2028041504 countsByYear W20280415042020 @default.
- W2028041504 countsByYear W20280415042021 @default.
- W2028041504 countsByYear W20280415042022 @default.
- W2028041504 countsByYear W20280415042023 @default.
- W2028041504 crossrefType "journal-article" @default.
- W2028041504 hasAuthorship W2028041504A5016119935 @default.
- W2028041504 hasAuthorship W2028041504A5023886894 @default.
- W2028041504 hasAuthorship W2028041504A5038194767 @default.
- W2028041504 hasAuthorship W2028041504A5039579571 @default.
- W2028041504 hasAuthorship W2028041504A5046154621 @default.
- W2028041504 hasAuthorship W2028041504A5069716975 @default.
- W2028041504 hasAuthorship W2028041504A5085424463 @default.
- W2028041504 hasAuthorship W2028041504A5087001996 @default.
- W2028041504 hasBestOaLocation W20280415041 @default.
- W2028041504 hasConcept C104317684 @default.
- W2028041504 hasConcept C181199279 @default.
- W2028041504 hasConcept C185592680 @default.
- W2028041504 hasConcept C201663137 @default.