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- W2028055334 abstract "Abstract Chinese hamster ovary cells were incubated in medium containing hypoxanthine and glycine and supplied with 10 −5 M methotrexate to inhibit the endogenous synthesis of thymidine nucleotides. Within 1–5 min after addition of the inhibitor, incorporation of [6- 3 H]deoxyuridine into DNA was as low as 1 to 1.5 per cent of control values, indicating that endogenous synthesis of thymidine nucleotides was blocked rapidly and almost completely. The cellular dTTP content was determined under various culture conditions as a function of time after addition of methotrexate. If the medium used contained undialyzed fetal calf serum, dTTP levels decreased relatively slowly in asynchronous as well as in synchronous S-phase cell populations. Similar results were obtained with asynchronous cultures incubated in medium with dialyzed serum. In contrast, if synchronous cultures consisting predominantly of cells in S phase were incubated in medium containing dialyzed serum and supplied with methotrexate, dTTP levels decreased within 15 min from control values of 15–20 pmoles/10 6 cells to levels of 1.5 to 3 pmoles/10 6 cells or lower. The previously reported failure of methotrexate to cause a rapid depletion of cellular dTTP may reflect, therefore, maintenance of thymidine nucleotide pools by cells that are not in the S phase and/or uptake by cells of thymidine present in undialyzed serum used for preparation of culture media." @default.
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- W2028055334 date "1979-06-01" @default.
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- W2028055334 title "Effects of methotrexate on thymidine triphosphate levels in Chinese hamster cell cultures" @default.
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- W2028055334 doi "https://doi.org/10.1016/0006-2952(79)90640-3" @default.
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