Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028112825> ?p ?o ?g. }
- W2028112825 endingPage "298" @default.
- W2028112825 startingPage "287" @default.
- W2028112825 abstract "The performance characteristics of multidimensional liquid chromatographic protein separations were evaluated using on-line electrospray mass detection, and a novel workflow for automated LC/MS data processing. Two-dimensional ion exchange/reversed-phase LC separations of Escherichia coli cytosol were conducted using either a continuous linear or discontinuous step gradient in the first dimension. Chromatographic profiles of the top 100 most abundant components were characterized to assess overall separation reproducibility within each mode, and to characterize differences in component distribution between the two modes of operation. Analysis of the resulting data indicates that multidimensional separations of complex protein mixtures can be done reproducibly. Furthermore, under the conditions employed within this study, a linear first dimension gradient was more effective at fractionating the protein mixture, distributing fewer major components to multiple second dimension cycles than an equivalent step gradient. The application of on line mass spectrometry, and automated processing of the resulting data, proved valuable for producing component level analysis of multidimensional protein separations." @default.
- W2028112825 created "2016-06-24" @default.
- W2028112825 creator A5010863240 @default.
- W2028112825 creator A5040885924 @default.
- W2028112825 creator A5041561546 @default.
- W2028112825 creator A5045247703 @default.
- W2028112825 creator A5056166716 @default.
- W2028112825 creator A5059848708 @default.
- W2028112825 date "2005-06-01" @default.
- W2028112825 modified "2023-09-25" @default.
- W2028112825 title "Evaluation of multidimensional (ion-exchange/reversed-phase) protein separations using linear and step gradients in the first dimension" @default.
- W2028112825 cites W1519955500 @default.
- W2028112825 cites W1966820357 @default.
- W2028112825 cites W1981925074 @default.
- W2028112825 cites W1982446250 @default.
- W2028112825 cites W1986242002 @default.
- W2028112825 cites W1988098839 @default.
- W2028112825 cites W1994540482 @default.
- W2028112825 cites W2002326985 @default.
- W2028112825 cites W2007286790 @default.
- W2028112825 cites W2022853146 @default.
- W2028112825 cites W2032509776 @default.
- W2028112825 cites W2035758756 @default.
- W2028112825 cites W2039738824 @default.
- W2028112825 cites W2040975732 @default.
- W2028112825 cites W2042832215 @default.
- W2028112825 cites W2043050470 @default.
- W2028112825 cites W2046557218 @default.
- W2028112825 cites W2047463020 @default.
- W2028112825 cites W2048775944 @default.
- W2028112825 cites W2049928191 @default.
- W2028112825 cites W2051810521 @default.
- W2028112825 cites W2052920249 @default.
- W2028112825 cites W2054380340 @default.
- W2028112825 cites W2056709373 @default.
- W2028112825 cites W2057348553 @default.
- W2028112825 cites W2058499147 @default.
- W2028112825 cites W2059950827 @default.
- W2028112825 cites W2063820532 @default.
- W2028112825 cites W2069348376 @default.
- W2028112825 cites W2073682446 @default.
- W2028112825 cites W2088694714 @default.
- W2028112825 cites W2093419657 @default.
- W2028112825 cites W2097548092 @default.
- W2028112825 cites W2120761767 @default.
- W2028112825 cites W2133494087 @default.
- W2028112825 cites W2140550847 @default.
- W2028112825 cites W2152645274 @default.
- W2028112825 cites W2153180430 @default.
- W2028112825 cites W2155868283 @default.
- W2028112825 cites W2167692458 @default.
- W2028112825 cites W4248391137 @default.
- W2028112825 doi "https://doi.org/10.1016/j.chroma.2005.04.048" @default.
- W2028112825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16038315" @default.
- W2028112825 hasPublicationYear "2005" @default.
- W2028112825 type Work @default.
- W2028112825 sameAs 2028112825 @default.
- W2028112825 citedByCount "17" @default.
- W2028112825 countsByYear W20281128252012 @default.
- W2028112825 countsByYear W20281128252013 @default.
- W2028112825 crossrefType "journal-article" @default.
- W2028112825 hasAuthorship W2028112825A5010863240 @default.
- W2028112825 hasAuthorship W2028112825A5040885924 @default.
- W2028112825 hasAuthorship W2028112825A5041561546 @default.
- W2028112825 hasAuthorship W2028112825A5045247703 @default.
- W2028112825 hasAuthorship W2028112825A5056166716 @default.
- W2028112825 hasAuthorship W2028112825A5059848708 @default.
- W2028112825 hasConcept C113196181 @default.
- W2028112825 hasConcept C121332964 @default.
- W2028112825 hasConcept C162356407 @default.
- W2028112825 hasConcept C168167062 @default.
- W2028112825 hasConcept C185592680 @default.
- W2028112825 hasConcept C186060115 @default.
- W2028112825 hasConcept C194451485 @default.
- W2028112825 hasConcept C202444582 @default.
- W2028112825 hasConcept C33676613 @default.
- W2028112825 hasConcept C33923547 @default.
- W2028112825 hasConcept C43617362 @default.
- W2028112825 hasConcept C86803240 @default.
- W2028112825 hasConcept C97355855 @default.
- W2028112825 hasConcept C9893847 @default.
- W2028112825 hasConceptScore W2028112825C113196181 @default.
- W2028112825 hasConceptScore W2028112825C121332964 @default.
- W2028112825 hasConceptScore W2028112825C162356407 @default.
- W2028112825 hasConceptScore W2028112825C168167062 @default.
- W2028112825 hasConceptScore W2028112825C185592680 @default.
- W2028112825 hasConceptScore W2028112825C186060115 @default.
- W2028112825 hasConceptScore W2028112825C194451485 @default.
- W2028112825 hasConceptScore W2028112825C202444582 @default.
- W2028112825 hasConceptScore W2028112825C33676613 @default.
- W2028112825 hasConceptScore W2028112825C33923547 @default.
- W2028112825 hasConceptScore W2028112825C43617362 @default.
- W2028112825 hasConceptScore W2028112825C86803240 @default.
- W2028112825 hasConceptScore W2028112825C97355855 @default.
- W2028112825 hasConceptScore W2028112825C9893847 @default.
- W2028112825 hasIssue "1-2" @default.
- W2028112825 hasLocation W20281128251 @default.
- W2028112825 hasLocation W20281128252 @default.