FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028115766> ?p ?o ?g. }

• W2028115766 endingPage "297" @default.
• W2028115766 startingPage "291" @default.
• W2028115766 abstract "Object. Systemic nicotine has been hypothesized to cause degeneration of the intervertebral disc which in turn decreases vascular supply to the disc through a cholinergic receptor—mediated process. Another possible mechanism may be through direct regulatory effects on disc cells. In this study, the authors tested the hypothesis that nicotine adversely affects nucleus pulposus cells by directly inhibiting proteoglycan synthesis and gene expression of type II collagen (Phase I study). They also assessed the hypothesis that nicotine inhibits the bone morphogenetic protein (BMP)—2-induced upregulation of extracellular matrix (Phase II study). Methods. Cells were isolated from nucleus pulposus obtained in rat lumbar discs and cultured on a monolayer. Media were treated with nicotine and/or recombinant human (rh)BMP-2 for 7 days. Sulfated glycosaminoglycan (SO 4 -GAG) in media was quantified using 1,9-dimethylmethylene blue (DMMB) assay. Gene assay of types I and II collagen, Sox9, and glyceraldehyde-3-phosphate dehydrogenase were quantified using reverse transcriptase—polymerase chain reaction (RT-PCR) and real time PCR. In the Phase I study, nicotine-treated (100 µg/ml) and nontreated cells were compared. The s-GAG production and messenger RNA (mRNA) of type II collagen and Sox9 decreased significantly in the nicotine-treated group. In the Phase II study, five groups were compared: 1) nontreatment; 2) rhBMP-2 only (100 ng/ml); and 3–5) with rhBMP-2 (100 ng/ml) and increasing doses of nicotine (1 [third group], 10, [fourth group], 100 [fifth group] µg/ml). The SO 4 -GAG production and mRNA of type II collagen and Sox9 decreased significantly in the groups treated with rhBMP-2 combined with 10 and 100 µg/ml of nicotine compared with the group treated with rhBMP-2. Conclusions. The results of this study raise the possibility that nicotine may contribute to the process of disc degeneration by a direct effect on the nucleus pulposus cells, possibly by antagonizing the effect of BMP-2." @default.
• W2028115766 created "2016-06-24" @default.
• W2028115766 creator A5001930247 @default.
• W2028115766 creator A5027835055 @default.
• W2028115766 creator A5029733514 @default.
• W2028115766 creator A5056081741 @default.
• W2028115766 creator A5077560828 @default.
• W2028115766 creator A5085019215 @default.
• W2028115766 date "2003-10-01" @default.
• W2028115766 modified "2023-09-27" @default.
• W2028115766 title "Inhibition of proteoglycan and type II collagen synthesis of disc nucleus cells by nicotine" @default.
• W2028115766 cites W1937940610 @default.
• W2028115766 cites W1963900159 @default.
• W2028115766 cites W1964178830 @default.
• W2028115766 cites W1965447052 @default.
• W2028115766 cites W1977241898 @default.
• W2028115766 cites W1983972225 @default.
• W2028115766 cites W1990153483 @default.
• W2028115766 cites W1996156837 @default.
• W2028115766 cites W2000358009 @default.
• W2028115766 cites W2004232044 @default.
• W2028115766 cites W2014172138 @default.
• W2028115766 cites W2014333033 @default.
• W2028115766 cites W2016521084 @default.
• W2028115766 cites W2021115256 @default.
• W2028115766 cites W2032255522 @default.
• W2028115766 cites W2038107706 @default.
• W2028115766 cites W2038161233 @default.
• W2028115766 cites W2046466286 @default.
• W2028115766 cites W2061418608 @default.
• W2028115766 cites W2068253453 @default.
• W2028115766 cites W2071256255 @default.
• W2028115766 cites W2071983551 @default.
• W2028115766 cites W2089262798 @default.
• W2028115766 cites W2093255789 @default.
• W2028115766 cites W2100040264 @default.
• W2028115766 cites W2107166657 @default.
• W2028115766 cites W2121661142 @default.
• W2028115766 cites W2122891461 @default.
• W2028115766 cites W2130733926 @default.
• W2028115766 cites W2142722173 @default.
• W2028115766 cites W2156376285 @default.
• W2028115766 cites W2169453571 @default.
• W2028115766 cites W4247637440 @default.
• W2028115766 cites W4296588288 @default.
• W2028115766 doi "https://doi.org/10.3171/spi.2003.99.3.0291" @default.
• W2028115766 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14563147" @default.
• W2028115766 hasPublicationYear "2003" @default.
• W2028115766 type Work @default.
• W2028115766 sameAs 2028115766 @default.
• W2028115766 citedByCount "26" @default.
• W2028115766 countsByYear W20281157662012 @default.
• W2028115766 countsByYear W20281157662013 @default.
• W2028115766 countsByYear W20281157662014 @default.
• W2028115766 countsByYear W20281157662015 @default.
• W2028115766 countsByYear W20281157662016 @default.
• W2028115766 countsByYear W20281157662020 @default.
• W2028115766 countsByYear W20281157662022 @default.
• W2028115766 countsByYear W20281157662023 @default.
• W2028115766 crossrefType "journal-article" @default.
• W2028115766 hasAuthorship W2028115766A5001930247 @default.
• W2028115766 hasAuthorship W2028115766A5027835055 @default.
• W2028115766 hasAuthorship W2028115766A5029733514 @default.
• W2028115766 hasAuthorship W2028115766A5056081741 @default.
• W2028115766 hasAuthorship W2028115766A5077560828 @default.
• W2028115766 hasAuthorship W2028115766A5085019215 @default.
• W2028115766 hasBestOaLocation W20281157661 @default.
• W2028115766 hasConcept C104317684 @default.
• W2028115766 hasConcept C105580179 @default.
• W2028115766 hasConcept C105702510 @default.
• W2028115766 hasConcept C126322002 @default.
• W2028115766 hasConcept C134018914 @default.
• W2028115766 hasConcept C142724271 @default.
• W2028115766 hasConcept C153074725 @default.
• W2028115766 hasConcept C153911025 @default.
• W2028115766 hasConcept C189165786 @default.
• W2028115766 hasConcept C197534660 @default.
• W2028115766 hasConcept C204787440 @default.
• W2028115766 hasConcept C2776164576 @default.
• W2028115766 hasConcept C2777755357 @default.
• W2028115766 hasConcept C2779335624 @default.
• W2028115766 hasConcept C2779547902 @default.
• W2028115766 hasConcept C2780550940 @default.
• W2028115766 hasConcept C2780644872 @default.
• W2028115766 hasConcept C2781013607 @default.
• W2028115766 hasConcept C3020332539 @default.
• W2028115766 hasConcept C55493867 @default.
• W2028115766 hasConcept C71924100 @default.
• W2028115766 hasConcept C86803240 @default.
• W2028115766 hasConcept C95444343 @default.
• W2028115766 hasConceptScore W2028115766C104317684 @default.
• W2028115766 hasConceptScore W2028115766C105580179 @default.
• W2028115766 hasConceptScore W2028115766C105702510 @default.
• W2028115766 hasConceptScore W2028115766C126322002 @default.
• W2028115766 hasConceptScore W2028115766C134018914 @default.
• W2028115766 hasConceptScore W2028115766C142724271 @default.
• W2028115766 hasConceptScore W2028115766C153074725 @default.
• W2028115766 hasConceptScore W2028115766C153911025 @default.

• next