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- W2028135717 abstract "To test the hypothesis that NK cells have a role in clonal stem cell growth we performed clonal stem cell assays on normal bone marrow cells (cells obtained from 4 leukemic children in remission and off treatment for at least 6 months). Ficoll-Hypaque separated bone marrow cells (1×105 or 2×105) were incubated in rabbit C' alone (control) or in rabbit C' plus monoclonal anti-human NK cell antiserum HNK-1 (Leu-7) for 60 min. After one wash in alpha medium, control and antiserum-treated cells were resuspended to an identical volume and plated in 1% methylcellulose with 2 I.U. of sheep erythropoietin. Erythroid and myeloid colonies were scored after 6-7 days culture. The HNK-1 treated cells consistently formed more erythroid and myeloid colonies than the cells treated with C' alone (control). These findings strongly suggest that removal of bone marrow NK cells increases the proliferation of both erythroid and myeloid precursors. This is consistent with the view that NK cells exert a physiological inhibitory effect on both erythroid and myeloid stem cell proliferation." @default.
- W2028135717 created "2016-06-24" @default.
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- W2028135717 date "1984-04-01" @default.
- W2028135717 modified "2023-10-18" @default.
- W2028135717 title "TREATMENT OF MARROW CELLS WITH ANTI-NK CELL ANTISERUM INCREASES CLONAL GROWTH OR ERYTHROID AND MYELOID COLONIES" @default.
- W2028135717 doi "https://doi.org/10.1203/00006450-198404001-00983" @default.
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