FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028169618> ?p ?o ?g. }

• W2028169618 endingPage "4950" @default.
• W2028169618 startingPage "4940" @default.
• W2028169618 abstract "The role of the phospholipid environment in modulating the activity of the rat brain synaptic plasma membrane (SPM) Ca2(+)-ATPase was investigated by its reconstitution into different phospholipids. Retention of activity of the solubilized Ca2(+)-ATPase depended on addition of exogenous phospholipids. As the cholate concentration used for solubilization of native SPM increased, a larger excess of exogeneous phospholipids, relative to membrane protein, had to be added to maintain optimal activity. Highest ATP-dependent Ca2+ transport activity was obtained when reconstitution was carried out in calf brain phospholipids (BPLs) followed by soybean phospholipids (SPLs) and the lowest in egg PC; reconstitution at a 40:1 weight ratio of exogenous phospholipids to native SPM protein resulted in ATP-dependent Ca2+ transport of 40.0 +/- 4.16, 23.4 +/- 8.48, and 11.54 +/- 2.31 nmol of Ca2+ (mg of protein)-1 (5 min)-1, respectively. Partial substitution of egg PC with BPLs led to an increase in the activity of the reconstituted Ca2+ pump. The highest ATP-dependent Ca2+ uptake was obtained when ratios of 15:25 or 10:30 egg PC to BPLs were used. Testing the individual phospholipids participating in the BPL mixture showed that addition of PS to egg PC led to a consistent increase in Ca2+ pump activity. Substitution of 50% of the PC with PS resulted in a 3.8-fold higher ATP-dependent Ca2+ uptake than that obtained in egg PC alone. No other phospholipid tested--PE, SM, or PI--had a similar effect. Increasing the proportion of PS within the BPL mixture above its original content led to a gradual decrease in the reconstituted SPM Ca2+ pump activity. Enrichment of asolectin with PS led first to increased Ca2+ pump activity; then, as the proportion of PS increased, Ca2+ transport of the reconstituted pump decreased. An increased proportion of PE, SM, or PI within the BPLs or asolectin, above their original contents, resulted in decreased Ca2+ transport. These results indicate that optimal SPM Ca2+ pump activity requires the combined presence of a critical amount of PC and PS within the reconstituted membrane." @default.
• W2028169618 created "2016-06-24" @default.
• W2028169618 creator A5077649431 @default.
• W2028169618 creator A5078237014 @default.
• W2028169618 date "1990-05-01" @default.
• W2028169618 modified "2023-10-15" @default.
• W2028169618 title "Role of the phospholipid environment in modulating the activity of the rat brain synaptic plasma membrane calcium ATPase" @default.
• W2028169618 cites W1132588258 @default.
• W2028169618 cites W1486949053 @default.
• W2028169618 cites W1513897103 @default.
• W2028169618 cites W1529235801 @default.
• W2028169618 cites W1536640324 @default.
• W2028169618 cites W1550032915 @default.
• W2028169618 cites W1554726437 @default.
• W2028169618 cites W1560215073 @default.
• W2028169618 cites W1588284427 @default.
• W2028169618 cites W1599011232 @default.
• W2028169618 cites W1775749144 @default.
• W2028169618 cites W1912410317 @default.
• W2028169618 cites W1964490459 @default.
• W2028169618 cites W1964671048 @default.
• W2028169618 cites W1973456800 @default.
• W2028169618 cites W1974141214 @default.
• W2028169618 cites W1984906315 @default.
• W2028169618 cites W1986299097 @default.
• W2028169618 cites W1986743999 @default.
• W2028169618 cites W1988161944 @default.
• W2028169618 cites W1988625231 @default.
• W2028169618 cites W1990267902 @default.
• W2028169618 cites W2002226892 @default.
• W2028169618 cites W2008324925 @default.
• W2028169618 cites W2019812624 @default.
• W2028169618 cites W2020220074 @default.
• W2028169618 cites W2034260980 @default.
• W2028169618 cites W2035025388 @default.
• W2028169618 cites W2041757099 @default.
• W2028169618 cites W2047695457 @default.
• W2028169618 cites W2051187845 @default.
• W2028169618 cites W2056640857 @default.
• W2028169618 cites W2063112617 @default.
• W2028169618 cites W2065447899 @default.
• W2028169618 cites W2072202987 @default.
• W2028169618 cites W2082890710 @default.
• W2028169618 cites W2085085025 @default.
• W2028169618 cites W2093331679 @default.
• W2028169618 cites W2111982547 @default.
• W2028169618 cites W2146916230 @default.
• W2028169618 cites W2175064241 @default.
• W2028169618 cites W2176475744 @default.
• W2028169618 cites W2179038293 @default.
• W2028169618 doi "https://doi.org/10.1021/bi00472a026" @default.
• W2028169618 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2163673" @default.
• W2028169618 hasPublicationYear "1990" @default.
• W2028169618 type Work @default.
• W2028169618 sameAs 2028169618 @default.
• W2028169618 citedByCount "20" @default.
• W2028169618 countsByYear W20281696182016 @default.
• W2028169618 crossrefType "journal-article" @default.
• W2028169618 hasAuthorship W2028169618A5077649431 @default.
• W2028169618 hasAuthorship W2028169618A5078237014 @default.
• W2028169618 hasConcept C12554922 @default.
• W2028169618 hasConcept C178790620 @default.
• W2028169618 hasConcept C181199279 @default.
• W2028169618 hasConcept C185592680 @default.
• W2028169618 hasConcept C198684833 @default.
• W2028169618 hasConcept C23265538 @default.
• W2028169618 hasConcept C2778918659 @default.
• W2028169618 hasConcept C41625074 @default.
• W2028169618 hasConcept C519063684 @default.
• W2028169618 hasConcept C55493867 @default.
• W2028169618 hasConcept C86803240 @default.
• W2028169618 hasConceptScore W2028169618C12554922 @default.
• W2028169618 hasConceptScore W2028169618C178790620 @default.
• W2028169618 hasConceptScore W2028169618C181199279 @default.
• W2028169618 hasConceptScore W2028169618C185592680 @default.
• W2028169618 hasConceptScore W2028169618C198684833 @default.
• W2028169618 hasConceptScore W2028169618C23265538 @default.
• W2028169618 hasConceptScore W2028169618C2778918659 @default.
• W2028169618 hasConceptScore W2028169618C41625074 @default.
• W2028169618 hasConceptScore W2028169618C519063684 @default.
• W2028169618 hasConceptScore W2028169618C55493867 @default.
• W2028169618 hasConceptScore W2028169618C86803240 @default.
• W2028169618 hasIssue "20" @default.
• W2028169618 hasLocation W20281696181 @default.
• W2028169618 hasLocation W20281696182 @default.
• W2028169618 hasOpenAccess W2028169618 @default.
• W2028169618 hasPrimaryLocation W20281696181 @default.
• W2028169618 hasRelatedWork W1969988449 @default.
• W2028169618 hasRelatedWork W1979156653 @default.
• W2028169618 hasRelatedWork W1997484252 @default.
• W2028169618 hasRelatedWork W2011867760 @default.
• W2028169618 hasRelatedWork W2089104225 @default.
• W2028169618 hasRelatedWork W2133255487 @default.
• W2028169618 hasRelatedWork W2150391754 @default.
• W2028169618 hasRelatedWork W2249456921 @default.
• W2028169618 hasRelatedWork W2334502792 @default.
• W2028169618 hasRelatedWork W2908325861 @default.
• W2028169618 hasVolume "29" @default.

• next