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• W2028175192 abstract "Enhanced fluorescence on silver island films (SIFs) is utilized to develop a sandwich-format immunoassay for the cardiac marker myoglobin (Myo). Myoglobin was first captured on surfaces coated with anti-Myo antibodies; the surface was then incubated with fluorescently labeled anti-Myo antibodies. The system was examined on glass surfaces and on SIFs. We observed the enhancement of the signal from SIFs in the range of 10–15-fold if compared to the signal from the glass substrate not modified with a SIF. A kinetic immunoassay for Myo on SIF-modified surface results in a decreased background signal. The initial results show that it is possible to detect Myoglobin concentrations below 50 ng/mL, which is lower than clinical cut-off for Myoglobin in healthy patients. We suggest the use of SIF-modified substrates for increasing the sensitivity of surface assays with fluorescence detection." @default.
• W2028175192 created "2016-06-24" @default.
• W2028175192 creator A5011159632 @default.
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• W2028175192 date "2005-07-01" @default.
• W2028175192 modified "2023-10-16" @default.
• W2028175192 title "Myoglobin immunoassay based on metal particle-enhanced fluorescence" @default.
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• W2028175192 doi "https://doi.org/10.1016/j.jim.2005.04.020" @default.
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